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Preparing red cell suspension for grouping

Kandahlawi
in Transfusion Services

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comment_67381

Hi, just wanna ask your practice in preparing red cells suspension for routine ABO blood grouping (pretransfusion testing) by tube method. In our SOP (reference: AABB Technical Method), we must wash the red cells 3 times before preparing the suspension. But in practice, we actually skip the wash step, and straight away proceed to suspense the cell in saline. We feel the wash step is a bit tedious. What do you all think of this practice? 

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comment_67382

Agree-

Our practice is to wash the cells 3 times if we perform DAT

For ABO/RH typing, we suspend cells with saline and proceed to typing.

comment_67384

PLease see my post and explanation on your other, identical, thread.

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