Hello,

We are looking at the Ortho Vision. However, it appears the Ortho QC is somewhat expensive at around $500 a month. At least here in Canada.

Has anyone had success with a home made recipe? We make our own QC in house for the Provue but am told it won't work with the Vision?

Appreciate your pros and cons on the instrument as well if anyone has a minute.

Thanks,

 

Richard

 

We use our own QC  from old patients.  AB+ and O=.

PRO: Free

CON: Hemolysis

This is even worse on the Vision as they have not fixed the problem with editting QC and Validating results, etc

 

You can now edit QC on the Vision.  You may also use User Defined QC.  Whole blood samples for Blood Bank and hemolysis can be problematic with any vendor or homemade creation.  The AlbaQ has less problem when stored in the upright position during storage, even the vials not yet in use.  It is very reliable and there's the ease of use with standard results and barcoding.

We made our own QC for Provue and had very minimal problems. I have continued to made my own QC for Vision with no problems as long as I am picky about the quality of plasma I pool and use. Can't have any hemolysis or lipemia. I do have the ability to edit QC results on the Vision and this is helpful of course. I know our rep and Ortho in general were very very pushy about buying their QC, saying it wouldn't work and "nobody does that". I beg to differ since about half of my training class DID in fact make their own. It is a huge savings to make your own for sure!

Sounds like you need a new rep, as ours has been very open about the ability to make up your own QC...

We just went live with our Vision and are purchasing the AlbaQ - it is expensive and I wasn't happy to learn the open expiry is only 7 days!

Will definitely consider making our own once we are settled in to the new instrument...now that I know this has worked well for others.

Does anyone have a procedure you are using to make your own QC? We’d like to stop using AlbaQ because of the expense.

Have 2 blood types that give a pos & neg reaction in each gel column, like the A neg and B pos of Albaq accomplishes.  For a 3 cell screen, an Anti D in a tube and an anti c in a tube.  Use plasma or a plasma like matrix, not just saline.  The number of drops of antisera depends on the strength or reactivity you want in your screens.

 

10 hours ago, ALLOURBESTNLR said:

Have 2 blood types that give a pos & neg reaction in each gel column, like the A neg and B pos of Albaq accomplishes.  For a 3 cell screen, an Anti D in a tube and an anti c in a tube.  Use plasma or a plasma like matrix, not just saline.  The number of drops of antisera depends on the strength or reactivity you want in your screens.

 

I would use plasma from patients that have produced those antibodies instead of antisera.   The commercial antisera is usually not IgG.  

R1R2 thank you! Do you have a formal policy and procedure that I can adapt and present to our medical director? I am a BB specialist so I want to make sure I’m doing this correctly.

ALLOURBRSTNLR thank you! Do you have a policy I can see to make sure I’m ding this correctly?

1 hour ago, Kerry said:

R1R2 thank you! Do you have a formal policy and procedure that I can adapt and present to our medical director? I am a BB specialist so I want to make sure I’m doing this correctly.

I am not a BBer and  don't have a policy to share but I am sure others here can share. 

we prepare our own QC for the vision, and it's working perfectly.. because sometimes the ortho confidence is not coming regularly and delay in the shipment occur.

our QC goes like this :

your will need a :

- patient sample in EDTA with blood group of O  and call it QC1

- patient sample in EDTA with blood group of B and call it QC2

- patient sample in EDTA with blood group of A and call it QC3

- patient sample in EDTA with blood group of AB and call it QC4

Regradless of the Rh, but one of them must be Rh negative and Du must be performed for detection.

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for ICT : the rule is to check every Panel 1 to 3 if they are working properly :

so it goes like this :

- place an anti-D in plan tube and mark it as QC5 = mostly will give you panel 1 and 2 ,,, so we need one addition antibody to test panel 3

- place 5ml a different antibody in plan tube as long as it's testing panel 3  this will complete the reaction of the ICT panel 1.2.3 and mark this and call it QC6

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For phenotyping

 - use a patient sample and use it as a Rh phenotype sample EDTA and if it could be K+ ag that would be better call it QC7

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Radwan1411...thank you!! I’m not sure what ICT stands for.

you are welcome ,, Indirect Coombs Test or Antibody screening