How many out there are using complement control cells?

I use them -  in gel using the buffered gel card and bottled anti-C3b,-C3d.

I use them - tube method.

We use C3 coated cells with our Anti-C3b- C3d reagent by tube method.

Same as David (gel)

Same as Rita - been using the Immucor product in tube for years. This has been a CAP requirement for a while now.

_{We use Immucor test tube method.}

CAP 7/29/2013

TRM.40210

DAT

Phase II

 

When performing an antiglobulin test with anti-C3 antiglobulin reagents, C3-coated red blood cells are used as a control in all negative antiglobulin tests.

NOTE:  Complement-coated red blood cells must be used to confirm all negative antiglobulin test results when the antiglobulin reagent used for testing has anti-C3 reactivity.  Tests found negative by tube methodology must be verified by obtaining a positive test result after adding C3-coated (control) red blood cells.  If a licensed blood typing system is used that does not require verification of negative test results using C3-coated red blood cells, an appropriate quality control procedure must be followed, as recommended by the manufacturer.  If a polyspecific antiglobulin reagent is used, refer to checklist item TRM.40200.

Evidence of Compliance:

• Patient records/worksheet documenting confirmation of negative antiglobulin tests