I can't find the reason we use Anti-D series 4 and 5 on the Echo? Why not just one? I understand they are different clones but that seems to be all I understand

Surely two clones is enough reason? As far as I am aware the second clone allows you to identify Du-s. There are two clones of A as well between full and confirmatory cards with some technologies.

There are a number of great articles discussing this topic. I'll post references later if someone else hasn't already done so.

They detect different epitopes of the D antigen.  Some D IV and D mosiacs may make anti-D if they are mistakenly typed as D Pos and transfused D pos.  Or not get RHIG post partum.  I'm looking for a reference for you.

Thanks for your response. I would appreciate the references if you all have them available.

If you have access to Transfusion journal, John Judd published an article comparing different anti-D reagents including which reagents will react with which partial D.  Sorry I don't have it at my fingertips.

Is there a chart somewhere which we can use to interpret RH results from Echo?

how to interpret RH with following results...Echo gave interpretation for first three ...

Eg. anti-D1 1+ and anti-D2 neg

      anti-D1 2+ and anti-D2 1+

      anti-D1  0*  and anti-D2 0

      anti-D1  C  and anti-D2  C---in this case ABO RH type gave C for all wells...

 

Thank you in advance....

Your package insert should explain the differences between those reagents.

Thanks for reminding me that I was going to post some references. Here are just a couple articles I've compiled talking about Rh stuff.

• Denomme GA, Wagner FF, Fernandes BJ, et al. Partial D, weak D types, and novel RHD alleles among 33 864 multiethnic patients: implications for anti-D alloimmunization and prevention. Transfusion 2005; 45:1554–1560.
• Flegel WA, Denomme GA, Yazer MH. On the complexity of D antigen typing: a handy decision tree in the age of molecular blood group diagnostics. J Obstet Gynaecol Can. 2007;29:746-52.
• Flegel WA. How I manage donors and patients with a weak D phenotype. Curr Opin Hematol 2006;13:476–483
• Flegel WA. Molecular genetics and clinical applications for RH. Transfusion and Apheresis Science 2011;44:81–91.
• Sandler SG, Li W, Langeberg AL, Landy HJ. New Laboratory Procedures and Rh Blood Type Changes in a Pregnant Woman. Obstet Gynecol 2012;119:426–8.
• Denomme GA, Dake LR, Vilensky D, Ramyar L, Judd WJ. Rh discrepancies caused by variable reactivity of partial and weak D types with different serologic techniques. Transfusion 2008; 48:473–478