I was wondering if anyone has tried the new Hemobioscience complement check cells. I know the complement check cells in the past have not worked all the time and was wondering if this product was any better.

 

Immucor's complement check cells work great in gel (3-4+) - they are abysmal in tube.  Have not tried the ones you mention - too pricey for me.

Edited November 22, 201311 yr by David Saikin

The reactions were weak to 1+ at best. Sticking to Immucor here.

I have been using them for about a year now. The reactions are usually 1+ when incubated at RT for 5 min

^{We have been using the Hemobioscience check cells for a year without any major issues.  Usually our complement check cells are 1+ by tube and 3+ to 4+ using the gel buffer card.     We use to use Immucor's complement check cells and had several very weak to non existent reactions for QC, since we don't use alot of Immucor, Immucor's quoted price for us was high compared to Hemobioscience/Ortho pricing.}

The Anti-C3d reagent I am using states to interpret the immediate spin reaction before you incubate? It is Monospecific for Anti-C3d, complement the majority of times needs to be incubated to be detected. I am trying to find out an explaination why perform the IS?

I would think that it is to make sure that the red cells are not sensitised with an IgM (or cold-reacting IgG). If they are, you could get a false positive result with your monospecific anti-C3d.

Since they are all too pricey (this item used to cost about $16 years ago ... until they became required by the CAP!) so we make our own ... every 2 weeks.  Sugar packet from the cafeteria, fresh donor cells from our therapeutic phlebotomy patients, saline and Alsever's Solution ... you do the math.

 

We run these as Pos (Anti-C3bd) and Neg (no reagent) controls each time we run the test (gel) to show that the reagent works for that test at that time.  (We don't run many Anti-Complement DATs.)

The Anti-C3d reagent I am using states to interpret the immediate spin reaction before you incubate? It is Monospecific for Anti-C3d, complement the majority of times needs to be incubated to be detected. I am trying to find out an explaination why perform the IS?

Does the direction insert require two centrifugations of the test, immediately and after incubation even if the immediate-spin test is positive?

We do the same as David. We only do DATs in gel.

We do the same as David. We only do DATs in gel.

 

Ortho has some of the cards available? Isn't it?

If the one they do not have what is your procedure?

Do you wash cells before making suspension?

Since they are all too pricey (this item used to cost about $16 years ago ... until they became required by the CAP!) so we make our own ... every 2 weeks.  Sugar packet from the cafeteria, fresh donor cells from our therapeutic phlebotomy patients, saline and Alsever's Solution ... you do the math.

 

We run these as Pos (Anti-C3bd) and Neg (no reagent) controls each time we run the test (gel) to show that the reagent works for that test at that time.  (We don't run many Anti-Complement DATs.)

Do yu mind sharing the SOP.  I think I will try to do this in my lab

 

Thanks