Okay, I'm looking for some advise as to a type discrepancy. I have a patient forward typing as an A, reverse typing as an O. Reacts with A1 lectin and A2 cells. The patient plasma was warmed to see if it could have been a cold and would warm away but there was still reactivity. Any suggestions?

Rouleaux or a strong anti M?

Have you tried a 'cold' panel? Also, what's the matter with the patient, have they been transfused, etc.

We are doing a cold screen and it is reacting 4+ at room temp and we are in the last stage of the screen. We are thinking a cold auto? We are also in the process of performing a panel to see if we can rule in or out anti-M but so far everything is reading 4+

What is the auto?........and, as John asks, what is the underlying pathology?

Also, try a panel at 30oC.

Sorry , also meant to ask, have you tried a DAT using a monospecific anti-C3d?

DAT negative

by auto I meant cold autoantibody

No history of transfusion no pregnancies

the full prewarm panel is incubating 37 degrees