I work in a lab that has been using bromelin to treat cells for cold autoadsoprtions for years.....but we have no references listed with the procedure and I can't find documentation of what was done to validate the process. Is anybody else using bromelin for this? If so, do you have any references for it? The package insert for Immucor Gammazyme-B makes no mention of its use for this purpose.

We don't use bromelin-treated red cells for this, but we do use papain-treated red cells, and they work just fine on most occasions - although, of course, when the titre of the antibody is really high (way in the thousands), it can take a few adsorptions!!!!!!!!!!!