Hi all,

I am currently trying to revise the antibody identification guidelines when one suspect a 'HTLA' like antibody. I know some reference lab out there try to identify the specificity some do not. Can anyone share their protocol? Thanks

We are extremely lucky in having a very large range of "HTLA" negative red cells, either in liquid form or frozen down for reference work, and so we always do our best to fully identify any such antibody.

Thanks, Malcolm.

If we don't identify the antibody, just know it has high titer low affinity, can we call it HTLA? I remember I have read some post says the HTLA we called before not always show the high titer and low affinity, if my memery don't cheat me.

Edited June 18, 201213 yr by shily
spelling error

Yes, you are correct in thinking that not all "HTLA" antibodies have "read the text books" and that some of them are of considerable affinity, or have a low titre (or both). This is why we try to get a difinitive specificity each time, if we possibly can (although, I have to admit that those antibodies that do not react as would be expected are few and far between).

For real HTLA antibodies, I am not affraid. I am affraid of those that mimic a HTLA and are a antibody that can cause a reaction.

The reason to specify a HTLA is that only then you are sure that it will not cause a reaction. Therefor weak reactive but with a high titer is not enough to clame a HTLA, there are lot of antibodies that can cause red cell destruction but mimic an HTLA (the Dombrock, Cromer, Lutheran, Cartwright, ...)

Peter

For real HTLA antibodies, I am not affraid. I am affraid of those that mimic a HTLA and are a antibody that can cause a reaction.

The reason to specify a HTLA is that only then you are sure that it will not cause a reaction. Therefor weak reactive but with a high titer is not enough to clame a HTLA, there are lot of antibodies that can cause red cell destruction but mimic an HTLA (the Dombrock, Cromer, Lutheran, Cartwright, ...)

Why at our transfusion service we send all this fun stuff to REFERENCE LAB (LOL).....

Why at our transfusion service we send all this fun stuff to REFERENCE LAB (LOL).....

Some fun for us!!!!!!!!!!!!!!!!!!!!!!!!

:haha::haha:

Yes, I agree some fun for us in reference lab but most of the time I feel like pulling my hairs trying to figure out the unknowns. Malcolm, I totalyl envious of your lab collections of those cells!!!!!

Yes, I agree some fun for us in reference lab but most of the time I feel like pulling my hairs trying to figure out the unknowns. Malcolm, I totalyl envious of your lab collections of those cells!!!!!

Yes, I am like a pig in clover, and I would like to publicly thank the staff for collecting these cells over the years, especially a guy named Alan Gray, who is in charge of screening for rare donors.