to run in parallel with EGA-treated cells when antigen typing red cells post EGA treatment as a control. I forgot the reason for doing this. Could someone remind me? I thought it's to check for polyagglutination when your treated cells expose to anti-T? but we're running this against manufactured antisera and not against patient serum (which has anti-T)....

Does the EGA product insert instruct you to run this control in parallel? It seems overkill as long as you have a single negative antigen typing result somewhere...and your post treatment DAT is negative

Thanks Angela. I think it's redundant too but it states in the technical manual. I think I'll just note to run that only if all Coombs results are positive, even if the DAT is negative.