From timt-to-time you do see cases where the auto is positive, but the DAT is negative, which would suggest an auto-antibody.

Please could you give us a little more detail, such as the lady's ethnic origin?

Have you tried enzyme treating the panels and the auto? It may just be that the antibody is an "auto-anti-D-like" antibody. It may also be worthwhile testing the lady's plasma against group O, rr, cord blood.

Malcolm, the autocontrol is so strong3+, and the DAT is neg, it is so unusual.

We have a couple of canned comments we use when the D is 2+ or less, to the effect that the patient may be a D variant and could benefit from RhoGam, and also to transfuse Rh negative. At least the doctor is aware of the problem, assuming they read the report.

Malcolm, the autocontrol is so strong3+, and the DAT is neg, it is so unusual.

I agree; it is extremely unusual.

why will i enzyme treat the panel & auto?the reactions are 3+ on my panel,isn't it that enzyme is just use to enhance or destroy rxn? if it is auto-antibody does my DAT suppose to be positive too?but i already did it twice & it is really negative. ethnicity is white female.thanks

why will i enzyme treat the panel & auto?the reactions are 3+ on my panel,isn't it that enzyme is just use to enhance or destroy rxn? if it is auto-antibody does my DAT suppose to be positive too?but i already did it twice & it is really negative. ethnicity is white female.thanks

Sorry, but someone has asked me not to comment on this one for the time being. Once I get the OK again, I'll reply properly.

why? any how I'm looking forward to your reply & thank you for the info.By the way,just an additional info,I run my panel using gel method.I x-matched O pos PRBC & it turned out to be strongly incompatible.

Malcolm, I am very interesting in your mystery, and I am expect the time you can tell us.

Before I know it, from my view I think maybe it is the antibodies that against the gel or things in it? And there are some anti-albumen show the specificity of anti-C.

This antibody maybe anti-gel or things in it but show specificity of anti-D or as you say anti-LW.

The way to solve it is to change other method to do the AD, such as tube or others.