Okay I am wondering what types of ernzymes everyone uses... we currently use Ficin/ Papain, and are looking at some others... What are your thoughts?

As a small hospital reference lab I only use ficin, but I do have WARM, which I use it off label.

Like David, I use ficin and WARM.

This may not be a lot of use to you if you cannot get hold of the book, or you want to know a bit more detail, BUT...

If you look on page 521 of "The Blood Group Antigen FatsBook" (should be in ALL blood transfusion laboratories - it's brilliant), by Marion Reid and Christine Lomas-Francis, 2nd edition, 2004, Elsevier Academic Press, there are all sorts of facts about the different enzymes used in blood transfusion.

That having been said, many of the different enzymes are only regularly used in very specialised laboratories, so be aware that you may be making a rod for your own back, as all will need to be validated, quality controlled, ...you name it!

:ohmygod::ohmygod:

Thanks!

Besides Immucor, where can you purchase papain or ficin?

I put "papain ficin suppliers" into my search engine and got 9060 hits, so there must be others out there.

I must admit that I did NOT look at every one of those 9060 sites!!!!!!!!!!!!!!!!!!!!!!

We order from Sigma Aldrich.