We just purchased two new Hettich EBA 21 serofuges and I'm wondering if anyone is willing to share your Implementation/Validation Plan with me so I don't have to re-invent the wheel. I'd really appreciate anything I could get my hands on. If you're willing to share, please e-mail to me at thintz@altru.org or fax to 701.780.1897.

Thanks in advance.

Terri, We have a generic plan that we just fill in with whatever we think is necessary for the equipment. Do you want that or are you looking for something more specific to the centrifuge?

I am looking into getting one of the EBA-21 centrifuges and would like to hear from someone who uses it. Currently we use a Clay Adams 2001. Our main problem is with the lid - it cracks around the hinge area . Unfortunately the lid cannot be replaced and I have to order a new centrifuge. They sure don't make 'em like they used to!!!!!

:sarcastic:sarcastic:sarcastic:sarcasm:

Edited May 26, 201015 yr by KKidd
can't spell!

One of the hospitals in our system uses them to spin specimens not for actually testing. They like it well enough. You do need to lightly lubricate the seal periodically (I think we specify monthly) in order to keep it from cracking.

:clap:We have 3 of them and like them very well. No problems since we purchased the 1 1/2 yrs. ago. Helmer sells them and was helpful in setting up the sppeds and slow down times.

We have been using 2 of them - first one since 2004. They are smooth and quiet, no problems with either of them. They do have a bit larger footprint than what we were using before. The older one has a flat top, which means that the buttons and readout are on the top and you can't see them very well from a sitting position unless you are tall (which I'm not). They improved them by putting the controls and readout display on a slanted 'bump' on top, much easier to see and use - which is how our newer one is set up.

Hello,

Would anyone be willing to share an SOP for the Hettich EBA 21 which includes the maintenance and QC? Searching for some ideas. Thank you so much!

I have 2 and I don't like them at all. They are not blood bank centrifuges in my opinion. The heads don't come out. However, they are quiet and are highly programmable. You have to set the brake speed to around setting 6 or the cells get mixed up when washing.

Edited June 1, 201015 yr by Trek Tech
incomplete sentence

Our two are model EBA 21 - their heads do come out. In fact, there are several different heads you can get for that model for a variety of purposes.

@clmergen: Thanks for the reply. I too have a general plan template but was looking for what someone has done specific to the EBA 21. I appreciate the offer though!

@ clmergen - do yo have an sop or even a draft that you could share for me to get an idea of writing our own sop for this centrifuge? Thank you!

Here is my generic procedure that also includes the EBA 21 requirements. (if it worked).

Serofuge, Immufuge and EB 21 Centrifuge Quality Control.doc

I validated a hettich for ABO/Rh back in 2008.

Here is our Validation Protocol and Validation Worksheet.

Hettich Validation Worksheet.doc

Hettich Validation.doc

We just purchased three new Hettich EBA 21 serofuges. While performing the calibrations we found that the cell button of the negative reactions do not form a very tight cell button and always seem to have a "tail". We have changed breaking speeds and accelerations speeds but neither helps. Anyone else have this problem? Thanks

We have the brake and accelerate at 9 for 20 second spins...No tails at all. However, we don't have the removable centrifuge heads.

We brake set at 6 to stop for washing ...any higher and the cells mix while braking.

Thanks we will try those settings. Do you use 20 sec spins for all phases? We still use tube method.

Yes, yearly calibration always yields 20 seconds all phases....

We spin 20 sec for all tests, with 9 set for acceleration and 5 set for braking - no tails.

We can't get our Hettich EBA 21 to do a good job of spinning tubes.  We find we get different answers from titers run using it. The cells get mixed up in a manual wash.  The vendor suggested we change settings so the braking is less but then it takes forever to stop.  My staff wants to get rid of them but there isn't much out there to buy these days.  Does anyone have a good answer?

We have had the Hettich EBA 21 centrifuges for years - have never had any problems with them.  Speed 3000 rpm, ramp up 9, ramp down 5, for all times (20, 45 and 60 secs).

We wash our titers in the Helmer Ultra CW, but spin them in 1 of 2 Hettich EBA 21s.  Within reason (and training), we do OK with titers (Cap proficiency is midrange  on the bell-curve.)