plz.. for the QC on washed LR-RBC'S

i need to know how to calculate the total protein after washing LR-RBC's ?? i need the equation.

and the amount of total protein post-washed ?

We don't perform any calculation. We just test the supernatant from the final product using the protein portion of a urinalysis dipstick to determine if the process removed "almost all of the plasma" (a quote from the Standards). We only accept products for release if the value is "negative" or "trace".

In the (very) old days, when we were making AHG from the raw serum of sheep, we had to get rid of the xenoantibodies by adsorbing with washed group O, group A and group B packed human red cells.

To test to make sure that the red cells had been adequately washed free of human plasma, so that the "good bits" of the AHG were not neutralised during this phase, we used to dilute some standard AHG in the supernatant (say 1 drop of neat AHG in 100 drops of the supernatant) to ensure that this did not happen (in other words, it was free of protein).

I am just musing, but I wonder if this could be a cheap and easy way of testing the amount of protein left in washed red cells?

It certainly work ed with AHG production and, although we never produced any figures, I'm sure that if it is "clean enough" not to ruin AHG, it's "clean enough" for the patient. Should we be quite so fixated with actual figures????????????

:confused::confused::confused::confused:

plz.. for the QC on washed LR-RBC'S

i need to know how to calculate the total protein after washing LR-RBC's ?? i need the equation.

and the amount of total protein post-washed ?

Why ( just showing lack of any Biochem knowledge here) can't you measure the total protein in each cycle of removed wash solution by running through an analyser. if an additional cycle is then run (that you would not normally perform), the difference between this and the previous wash would show how much protein was still around in the volume of blood washed?...........this may be totally wrong-i'm just brainstorming!!

Because most blood centers don't wash enough RBCs to justify the cost of an instrument when urinalysis dipsticks are so cheap.

Because most blood centers don't wash enough RBCs to justify the cost of an instrument when urinalysis dipsticks are so cheap.

Thanks Marilyn-I keep forgetting this isn't a hospital setting!