^^Yes, thanks Dr. Pepper--we have PEG, but I think we only use it as an enhancement for panels run via tube method. I'll have to dig around a bit in our procedures (which are old, outdated, in need of re-write. . .. Ugh!)

^^Yes, thanks Dr. Pepper--we have PEG, but I think we only use it as an enhancement for panels run via tube method. I'll have to dig around a bit in our procedures (which are old, outdated, in need of re-write. . .. Ugh!)

Oh no, you must review and/or revise annually or even sooner as the case presents itself in order to keep your accreditation. Importantly, your Medical Director must also review and sign to that effect annually. If you get a new director s/he must review all your SOPs within 6 months.

God Luck.

Liz

^^Yes, Liz--we are in dire straits when it comes to the procedure manual, sad to say. There's no standardization because techs are doing things in ways they've learned from the 2 or 3 supervisors we've had since the manuals were written, and nothing's been updated. I'm prepared to assist in rewriting them, but I need to get everyone's help in making notes and revising. Not an easy task when we to do our own work plus cover for the blood bank supervisor position that's still vacant--there's only so many bodies around in the lab, and we're a bit under the gun because we're due for inspection in the spring.

Wow, some problem.

Have inspected in the past - pick an employee, have the manual on my knee and ask - how do you do (say an auto-absorption). If it disagrees with what is in the manual, it would be cited. Also need evidence of regular updates and quality improvement and evidence that all staff have signed off on methods as having read them.

I remember the steep hill of when regulation came in years ago - daunting, but when done, is easy to maintain.

Good luck with it all.

Cheers, Eoin

^^Yes indeed! I don't know how we've made it through the past few inspections in there with the manuals the way they are. I've inspected, too, and as long as you "do what you say, and say what you do", you're good. We're not good. . . . .

Good luck anyway.

Remember - lots of citations may bring more resources.

Cheers

Eoin

Judd's Methods in Immunohematology has a section on cell separation with several methods. I like the centrifugation method. Simple and inexpensive to perform. Usually works pretty well.

Thank you, AMcCord.

Liz

Thank you, AMcCord.

Liz

You're welcome...

Originally Posted by Mau Feitio

The truth is where I work technicians always complaint about the adsortion and eluition techniques. Spend hours and hours with this patients and don´t give straight answers about the outcome of this studies. We use gel in our routine and I believe that it was lost the habit of working in tube by the youngsters. They seem to have difficulties on choosing the right paths during those studies. wonder if you could give me some suggestions on literature to help me creating a SOP where the work out strategy could be established. The real doubt is do you use always the same strategy on this cases? Could you help me out. Tx

I'll do my best, but we are being audited this week (AGAIN!) and so it may not be until next week.

If I should forget, please feel free to badger me!!!!!!!!!!!!!!!!

:D:D:D:D

This is it Malcolm: your documented promise.

PS: Hope you did well on your audit.

Thanks

We used to do lots of warm autoadsorptions. As far as procedure, it pretty much followed package insert of enzyme/DTT reagent we used. We had a large population of chronically transfused patients with lots of antibodies. Our particular patient population often had lower retic counts. We finally started using molecular typing and pheno matched cells as much as possible. Rarely did autoadsorptions or alloadsorptions after a point. Not in technical area now, so don't know if anythings changed or not.

We used to do lots of warm autoadsorptions. As far as procedure, it pretty much followed package insert of enzyme/DTT reagent we used. We had a large population of chronically transfused patients with lots of antibodies. Our particular patient population often had lower retic counts. We finally started using molecular typing and pheno matched cells as much as possible. Rarely did autoadsorptions or alloadsorptions after a point. Not in technical area now, so don't know if anythings changed or not.

What is your turn around time?

And x how much has the cost gone up.

Was the molecular typing done in a hospital based BB?