What are folks out there using for DAT controls for the ProVue?

We do not have Provues in Australia but we have the DiaMed Gel Express/Diana WA systems here that are equivalent but use the DiaMed brand gel cards. We actually do not have one in our labs but the principles of QC are the same for manual methods. Use a negative DAT cell and a weak positive. We make our own using a monoclonal IgG Anti-D coated onto R2R2 cells. We can make this very weak and very consistently. Unfortunatley the IgG Anti-D is not commercially available.

I hold the opinion that the commercially available C' coated cells or home made "cold Frutistone coat" C' coated cells are more problematic than helpful as the procedure is unreliable. We perform this method very often and use it to QC monoclonal anti-C3d but only because we have to. It is unreliable with monoclonal anti-C' and when it fails we have to re-make the cells and retest until it works. I think this makes it a very poor QC material and a poor QC material gives poor QC.

We do not have a provue; but we are using ortho gel.

This is what ortho told us to do for qc.

--for IgG card; use IgG coated check cells for positive control and a screening cell for negative control

--for IgG/C3d card: use IgG coated check cells for pos IgG control, use C3d coated ceck cells for positive

C3d, and use a screening cell for negative control.

All cells should be diluted to 0.8%.

We use O positive pooled patients who have a negative antibody screen. We set up two tubes, one with Anti-D added and the other without anti-D added. We test both our IgG and IgG/C3d cards against this.

This is what my Blood Bank Technical Specialist told me to do when I was validating our ProVue.

Using either the #4 AlbaQ-Chek control OR any Rh positive blood sample that you have, follow this procedure:

1. Spin down the sample and remove the plasma

2. Add 3-4 drops of Anti-D reagent and mix

3. Incubate at room temp for about 5 minutes

4. Wash one time with saline to remove excess anti-D

5. Return plasma to sample and spin down again.

This has worked well for us. It gives about a 3+ reaction for the DAT control

We do the same as Nancy F.

This is what my Blood Bank Technical Specialist told me to do when I was validating our ProVue.

Using either the #4 AlbaQ-Chek control OR any Rh positive blood sample that you have, follow this procedure:

1. Spin down the sample and remove the plasma

2. Add 3-4 drops of Anti-D reagent and mix

3. Incubate at room temp for about 5 minutes

4. Wash one time with saline to remove excess anti-D

5. Return plasma to sample and spin down again.

This has worked well for us. It gives about a 3+ reaction for the DAT control

Ortho told us to do this, as well. When I asked how they QC the complement portion of the Poly card, she was silent for a minute and then said...oh we don't QC the complement portion. She suggested I make complement coated cells (which ain't gonna happen!) or figure out the dilution of the commercially available complement check cells.

We decided to not do the DAT on the ProVue instead.