We do a forward ABO group and Rh type on all Rh negative Mom"s and O Mom's. For positive DAT's we do the LUI freeze technique for eluate for ABO incompatiblity. No other antibody screening is done if the LUI shows an ABO incompatibility. If the eluate is something other than an ABO antibody, then we would proceed with an antibody screen on maternal serum. It has been my experience that the Lui technique is not of much use except for ABO issues.

thanks,

John

Thank you John but how do you proficiency test your LUI?

We have another facility in the area and we trade samples every 6 months and use that as our proficiency samples. CAP has always approved this as a way of proficiency since I don't think it is offered in their kits. If it were not for the fact that another local facility does the LUI, then we would have to re-evaluated doing the test.

Thanks,

John

We call all positive DATs to the nurse taking care of the baby. The physicians then order a CBC and Bili and watch the baby and treat as needed.