Hello!

i'll like to know, what technology are you using for blood typing, red cell antibody screening and crossmatching??

the good, the not that good, and the bad about them!

thanks!

I use gel for everything I can. Tubes are used for IS xm, rosette tests, titers, sometimes abid's. I like the small volume of reagents used, the ease of reading (esp with all my techs being generalists) - this includes consistency of reaction strengths between techs, stat ABIRhs. Downside is that geling is a tad longer to perform and a bit pricey (though the vendors are working to make classic testing at least as costly as the newer technologies).

Edited August 28, 200915 yr by David Saikin
added more info

Is out there, any one, using Diamed gel??

is it FDA approved???

not in the USA, yet

Is out there, any one, using Diamed gel??

is it FDA approved???

The Red Cell Imunohaematology arm of the NHSBT in England uses DiaMed technology extensively (although not exclusively) and we are extremely impressed with it, for similar reasons to those given by David (although, of course, all of our staff are spacialists, rather than generalists).

That having been said, we do also use other technologies.

We will fall back on tube techniques on a fairly regular basis (about once or twice a day) when we are dealing with a case of AIHA, particularly if the autoantibody is "cold-reacting".

Thank you Malcolm!

I have another question!, Using Donor Grouping card (Diamed´s), they come with a reactive for D, that detects D(VI+).

so when u have a negative reaction, do u confirm it with convetional protocols for negative D in donor, or you just asume it´s a real negative, reporting and labeling the RBC as Rh negative?

No. The Testing Department does use an anti-D that detects Partial DVI, but they use something called Totem that is capable of detecting a wide range of other Partial D types, many of which would not be detected by "conventional" anti-D reagents. The Testing Departments do not, by the way, use DaiMed technology for their ABO and D typing. They use microtitre plates (I think).

Any "strange" reactions they get with their anti-D reagents automatically means they hand the donor samples over to us in RCI for complete elucidation (although we cannot claim to always get the answer; we sometimes have to send samples off to the International Blood Group Reference Laboratory for molecular work-ups).

Is out there, any one, using Diamed gel??

is it FDA approved???

I thought that Provue Gels used in the U.S ( and therefore FDA approved) were the same as the Diamed gels?