Sorry for my english and all my mistakes...I work in a small transfusional center in Italy. In my laboratory we have a WADIANA and we use it only for type and screen and when is necessary for cross match tests.Before WADIANA we did the cross match tests in tube.Our t&s is particular because we do also a DAT to every patients.At this moment T&S is valid only for three days for all type of patients .. I'll be courios to know how is T&S in your transfusional centers ... muchas gracias:)

Hi Skyanto,

Are there specific guidelines you follow in Italy that mentions what is good practice?

Sorry for my english and all my mistakes...I work in a small transfusional center in Italy. In my laboratory we have a WADIANA and we use it only for type and screen and when is necessary for cross match tests.Before WADIANA we did the cross match tests in tube.Our t&s is particular because we do also a DAT to every patients.At this moment T&S is valid only for three days for all type of patients .. I'll be courios to know how is T&S in your transfusional centers ... muchas gracias:)

Hello skyanto,

Rashmi is correct that you should see if there are any Guidelines specific to Italy, however, EU Regulations suggest that all criteria used within the EU should be the same (they are not, by the way).

The British Committee for Standards in Haematology Blood Transfusion Task Force produced Guidelines for the UK in 2004. The title is "Guidelines for compatibility procedures in blood transfusion laboratories" and deals with your queries in sections 4.6 and 4.7. The original was published in the British Blood Transfusion Society's Journal Transfusion Medicine in 2004 (volume 14, pages 59-73). If you cannot get hold of this journal, you can go onto the internet, put "BCSH Guidelines" into your search engine to get their website, then go to guidelines, blood transfusion and there you will find this particular guideline, which can be downloaded or printed in a PDF form.

It must be remembered that these are only guidelines, and that there are hospitals that do not follow them, but not to follow them is foolish in the extreme unless a thorough risk assessment has been carried out, documented and the risk found to be acceptable.

As I say, this guideline only applies to the UK, but it could be worth a read.

I hope this is of help to you.

:)

I don't know...I don't know who decide in Italy.. I have to ask to my head physician.... tomorrow I'll ask to him.. muchas gracias Rasmi.

Hello Skyanto

Ministero della Salute Decreto 3 Marzo 2005: Caratteristiche e modalità per la donazione del sangue e di emocomponenti. Art. 13. 3 (Gazzetta Ufficiale N.85 del 13 Aprile 2005. Internet link - http://gazzette.comune.jesi.an.it/2005/85/3.htm)

" Il campione deve essere raccolto in provetta sterile entre 72 ore precedenti al trasfusione....Se il paziente è stato trasfuso di piú di 4 settimane, o non è mai stato trasfuso, il campione puó essere raccolto entre i 7 giorni precendenti la trasfusione."

There is no mention of the Direct Coombs test, but it makes sense if the patient has been recently transfused to make sure that he hasn't made antibodies to the transfused blood

As far as I know there is no more recent 'Decreto' - but I might be wrong

galvania, that's just showing off!!!!!!!!!!!!!!!!!!!!!!!!!!

What does it mean, for those of us who can only just speak English and have trouble with that if there are more than a couple of syllables?????????!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!

When T&S is ordered by physician, we do

(1) ABO grouping and Rh typing.

(2) Antibody screen for alloantibodies that are madatory to screen, for example E, e, D, C, c, Jka, Jkb, Fya, Fyb, K, k, MNSs, P1, Lea, and Leb. Others depend on your population like Mia / MUR in Asia.

(3) Review and compare the results with patient past history, if any.

(4) Check if patient has clinically significant alloabs detected in the past.

(5) Any untow or NFHTR.

(6) Check if patient needs any specific blood products, etc.

I agree with Rashmi and Malcolm that you should consult your blood bank director or blood bank pathologist what guidelines you should follow, Do you have SOP in your laboratory?! Every med tech in your laboratory should follow the same guidelines and do the same thing.

CK Cheng, MSc, SBB(ASCP), CQA(ASQ)

Hong Kong

July 9, 2009

Thank you very much Galvania :)

I've read the "decreto" and I've found a lot of interesting informations..

I printed it this morning at work and all my friends were interested on it..

Hello ckcheng im my laboratory we haven't written guidelines about T&S..we started wiyh T&S at the end of 2008.For us T&S is perform a DAT,IAT and a control group;it's valid for three days and if a patient needs more transfusions during the three days we do only a control group. We have a lot of patients with DAT positive..

ckcheng what are SOP?

BUEN FIN DE SEMANA A TODOS

SOP stands for Standard Operation Procedures which is an approved set of written procedures that delineated in detail the procedures, policies, and processes perfromed in a blood center or hospital blood bank. Usually SOP is written by blood bank supervisor and validated by blood bank pathologist or director. All med tech working in blood bank should read, and understand the SOP, trained , and passed the proficiency test before doing test.

Here is an example of Tube Test for Rh Typing SOP

Principle:

Brief description of Rh and testing method. You may find info in most test books, or packing insert.

Reagents:

What reagent you are using? Polyclonal, monoclonal, or blend. What is the control? Size of tube, centrifuge, pipette, ......

Procedures:

Step by step decribe how you perform the test.

Interpretation:

What make you determine it is positiive, negative, and test is invalid.

Notes:

Are there any particulars things you want your staff pay attention? If there is, you may write it down here.

References:

Where are the information come from? Please cite it here.

Who prepared the SOP, and who validate the SOP. And when.

When you need to re-review it.

Every single test you peform in the laboratory should have an SOP.

Good sources are:

(1) Methods section in Technical Manual, AABB

(2) Methods in Immunohematology by John Judd, AABB

(3) Immunohematology Methods by American Red Cross

Hope that helps.

CK Cheng, MSc, SBB(ASCP), CQA(ASQ)

Hong Kong

July 11, 2009

galvania, that's just showing off!!!!!!!!!!!!!!!!!!!!!!!!!!

What does it mean, for those of us who can only just speak English and have trouble with that if there are more than a couple of syllables?????????!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!

Sorry Malcolm - not trying to show off; but as Italian guidelines were asked for, they are naturally printed in Italian............Sorry also that I took so long to reply - I was back in the UK for a few days, reminding myself of what the NHS is like (not for me - for my mum..) and had no internet access.

Translation is as follows:

Ministero della Salute Decreto 3 Marzo 2005: (Decree of the Minister of Health 3.3.2005

Caratteristiche e modalità per la donazione del sangue e di emocomponenti. Guidelines relating to the donation of blood and blood components

Art. 13. 3 (Gazzetta Ufficiale N.85 del 13 Aprile 2005. Internet link - http://gazzette.comune.jesi.an.it/2005/85/3.htm)

" Il campione deve essere raccolto in provetta sterile entre 72 ore precedenti al trasfusione....Se il paziente è stato trasfuso di piú di 4 settimane, o non è mai stato trasfuso, il campione puó essere raccolto entre i 7 giorni precendenti la trasfusione."

The sample must be collected into a sterile tube up to 72 hours before transfusion...If the patient has not been transfused in the last 4 weeks, or has never been transfused, the sample may be drawn up to 7 days prior to transfusion

Thanks Anna.

I was only joking about showing off!

:eek:

I do hope your mum is okay.