Hello All!

We currently do DAT's in tubes, Polyspecific first, and then if it is Positive, we will do IgG and C3d separately.

I would like to switch to doing DAT's in gel, because of the increased sensitivity (and we just got 3 ProVues). This presents a problem, however, because Ortho does not have a C3d gel card.

How many people out there are just doing IgG and Polyspecific only and letting the physician figure that if Poly is Positive and IgG is negative, it's probably due to Complement??? This is what I want to do, so we don't have to keep d oing the C3d portion in tubes, but I can't find any documentation in the Tech Manual to support this.

Any recommendations???

Thanks

Denise

Hi,

We started doing DAT in gel almost a year ago.

We call the gel portion of the test a sensitive "DAT screen". The gel is a combination of the IgG and c3.

If negative the test stops there.

The positives reflex to both the IgG portion in gel and the C3 test in tube.

We result as either positive for the complement portion, positive for the IgG portion, positive for both or negative.

The docs are suppose to understand that the screening is more sensitive and a final negative is not out of the question.

We use the buffered gel card to do the anti-complement testing . . . the immucor complement check cells are always 4+.

Hi Dave,

I see how you would add your 0.8% patient cells and your Anti-C3d to the buffered gel card, but how do you do your check cells? I guess I'm confused about that part.

Denise Campbell/Dufault

PS Are you still at Littleton Regional?

Hello All!

We currently do DAT's in tubes, Polyspecific first, and then if it is Positive, we will do IgG and C3d separately.

I would like to switch to doing DAT's in gel, because of the increased sensitivity (and we just got 3 ProVues). This presents a problem, however, because Ortho does not have a C3d gel card.

How many people out there are just doing IgG and Polyspecific only and letting the physician figure that if Poly is Positive and IgG is negative, it's probably due to Complement??? This is what I want to do, so we don't have to keep d oing the C3d portion in tubes, but I can't find any documentation in the Tech Manual to support this.

Any recommendations???

Thanks

Denise

Ortho BioVue do have Anti-IgG/Anti-C3b, C3d/Control cards (at least in Europe). We routinely use them.

Yes, I know. We do use the Anti-IgG/Anti-C3d, but cannot get the C3d/Control cards yet. But unfortunately, they are not available in the US yet. Thanks for your reply.

I assume the c3d check cells are run in gel in a separate well from the patient test. In tube testing we run check cells to make sure washing was adequate; since there is no washing in gel, it seems to me we should only have to run the check cells once each day of use, to prove the anti-c3d reagent is active.