Could anyone share what they use as an Rh control when doing tube testing? We only incorporate an Rh control when we have an AB person.

Thank you

We use Gammaclone control. (Our typing reagents are all Gammaclone.) You can use 6% albumin and I think some use saline.

we use 6% albumin for Rh control.

Do you dilute your own 6% albumin or can you buy this product?

Thanks

We generally use a 6% albumin solution that we prepare from 22% albumin. Patient's serum can also be used or any commercial antisera that gives a negative reaction. For AB Pos patients we generally use the 6%albumin or serum.

Do you dilute your own 6% albumin or can you buy this product?

Thanks

We prepare 6% from 22% albumin.

We also use 6% albumin diluted from the 22%.

We also use 6% albumin diluted from the 22%.

When you dilute it yourself what do you use for the expiration date? Does it have to be made every day or can it be used until it is all gone? What do you use as the lot number? We currently buy Ortho Rh control that is more than $1000 a box so we want to change to making our own. Thanks for your help!!!

Saline is our choice for Rh control, and economical.

You really should use a proprietary Rh control. The control should be EXACTLY the same formulation as the anti-D (minus the antibody, of course!) - not only with respect to albumin concentration, but other components of the buffer as well. Otherwise you could be missing false positives due to spontaneous agglutination/aggregation with one of the buffer components

Anna

Agree wholeheartedly with Galvania.

Inspectors over here would insist on the same formulation for Rh control (only minus Ab). This thread goes back to 2005 - interesting. Are we under pressure to do it "on the cheap"?

Regards

Eoin

Edited April 23, 200916 yr by Eoin

For those of you who use the Ortho Rh control, be aware that this control is only for the Ortho human based anti-D. It is not for the Ortho monoclonal anti-D. If you are using the monoclonal anti-D, you should use 6% albumin, saline or the patien'ts serum/plasma for the control.

We use a Rh control (6% albumin) only for AB positive patients when they are typed manually. We use the Ortho ProVue. So all patients typed on that have a Rh control as part fo the typing.

I would love to use a proprietary Rh control but I don't see that Ortho offers one for their BioClone Anti-D tube test. If I'm missing something please let me know

Sandy, You are not missing anything. Ortho does not have a Rh control for their Bioclone anti-D.

Galvania and Eoin are quite correct in my opinion.

If you do not use such a negative control, but use things like saline, albumin, saline and albumin, etc, you are not using a negative control; you are using a sop (and I don't mean a Standard Operating Procedure!) to make you think you are using a negative control. It gives you peace of mind when, in reality, you should be extremely nervous!