Hi everyone, a colleague needs an SOP for the titration of hemagglutinins for LTOWB. Could you please share your SOPs?

Thanks in advance for all your help.

This is just my opinion.

First, it's usually not a titer but a single-point dilution.

What dilution?  It's truly arbitrary.  Some go 1:50, some 1:100, others 1:200.  It depends on what the facility medical director will accept.

Your colleague should collaborate with their medical director to determine what they feel is "safe".  This cut-off will not prevent reactions, only minimize the risk.

Then from there, it should be a simple procedure of a single-point dilution, with a cut of 1+, 2+... or less.

Our supplier titers all our WB and it's marked with </=200. We only go "farther" and do an extra dilution for Oneg LTWB that we set aside for pediatric trauma use - it needs to be <50