Have people ever had to rule in or rule out cold antibodies based on reactions you get from a gel card panel/screen? 

For example in a case where you get a discrepant ABO result because a cold antibody is messing up the reverse typing. 

Anyone with any knowledge on this?  Thank you. 

Edited April 21, 20223 yr by sbbguy
brevity

Anti-M comes to mind most often, esp w Ortho gel cards.  It is possible to work up cold abs.  I've used the buffered gel cards to do so.   I've also used 2 stage papain pretreatment of panel cells (in gel). 

Edited April 21, 20223 yr by David Saikin
added more info

Gel likes colds, the larger IgM antibodies are more easily trapped in the columns matrix. You can only really identify cold antibodies with specificity (lots of anti-M) using Gel. For Panagglutination in screen, panel or a discrepant back type we resolve using tube.

We regularly used to run RT or even cold gel panels (LISS cells on Saline cards) to id M, P1, Le etc.

If suspected A2 patient with anti-A1, use A2 cells in the reverse grouping card.

We would test the grouping cells for the identified antibody.

WARNING! These may require a degree of skill as may have to perform manual testing.