For anyone who is washing red cells, what do you use as your quality control criteria for the final product? Is it based on any standards? I have looked at AABB standards but cannot find anything specific there. What is the HCT level you are aiming for? What is the protein level you are aiming for? Any other criteria?

We use COBE 2991s, and use protein dipsticks to test the supernatant. The positive control is diluted plasma and we dilute it to get a level of 30, the negative control is saline, and the samples are collected from the wasteline after the washing is done. The washed sample should test for negative or trace protein, following Standard 5.7.4.6, which lists that washed cells should be prepared in a way that removes almost all of the plasma. We don't look at the crit for these.

There are some other threads on here that also discuss washed QC -- I would also search for those!

 

This is going back donkey's years, but when we used to adsorb raw AHG with human red cells to get rid of the xenoantibodies that reacted like anti-A and anti-B, we used the last supernatant to dilute an aliquot of a previous lot of AHG, and then saw if the AHG still worked.  If it did, then the red cells were washed sufficiently free of human protein, which, otherwise, would have inhibited the AHG.