We got a blood sample for ABO/Rh and Antibody screen testing. 

Results: 

Forward typing: 

Anti- A : Negative

Anti-B : Negative

Reverse typing: 

A1 cells: 4+

B cells: 4+ 

 

Antibody screen and auto-control:

Cell 1: 4+

Cell 2: 4+

Cell 3: 4+

Auto-control: Negative

Patient is from Mumbai. 

 

 

Looks like it could be an Oh individual.

Try testing the red cells of the patient with Ulex europeaus.

20 minutes ago, Malcolm Needs said:

Looks like it could be an Oh individual.

Try testing the red cells of the patient with Ulex europeaus.

 

Result:

Patient red cells + Ulex europepeaus = Negative 

or there are unexpected antibody/antibodies in the patient's plasma.

Edited May 18, 20205 yr by yan xia

Have you attempted an antibody identification panel?  

56 minutes ago, yan xia said:

or there are unexpected antibody/antibodies in the patient's plasma.

 

That is what we are trying to figure out. Thank you

4 minutes ago, John C. Staley said:

Have you attempted an antibody identification panel?  

 

Result:

Antibody identification panel:

All panel cells = 4+

Auto-Control= Negative

 

 

What you are identifying is almost certainly a strong anti-H in an Oh individual.  However, if the individual requires a transfusion, you will need to perform differential allo-adsorption (or something similar) to identify any other underlying clinically significant atypical antibodies (you can ignore any underlying Lewis antibodies, which are commonly also present).

Could have Anti-Jk3?

3 hours ago, jojo808 said:

Could have Anti-Jk3?

Why would the red cells of an individual who is Jk(a-b-) not react with Ulex europeaus?

On 5/18/2020 at 2:46 AM, diplomatic_scarf said:

We got a blood sample for ABO/Rh and Antibody screen testing. 

Results: 

Forward typing: 

Anti- A : Negative

Anti-B : Negative

Reverse typing: 

A1 cells: 4+

B cells: 4+ 

 

Antibody screen and auto-control:

Cell 1: 4+

Cell 2: 4+

Cell 3: 4+

Auto-control: Negative

Patient is from Mumbai. 

 

 

What is the patient's Rh type?

I agree with Malcolm,  definitely looks like anti-H.

6 hours ago, LK11 said:

I agree with Malcolm,  definitely looks like anti-H.

And we need to remember: patient is from Mumbai.

On 5/19/2020 at 6:57 AM, jayinsat said:

What is the patient's Rh type?

 

Anti-D + patient's red cells : 4+ 

 

12 hours ago, diplomatic_scarf said:

Anti-D + patient's red cells : 4+ 

I can't say that I am in the least surprised about this, given the patient's ethnicity.  There is a frequency of the D antigen of 99% in most Asian populations.