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comment_78398

Currently our lab ignores +/-, 0.5, or weak positive results (depending what your technology classes them as) on titres, hence we only report the titre as the value that gives a minimum 1+ reaction. Is this usual practice? recently we are getting alot of +/- Anti-D's that are only giving very weak reactions in IAT but confirmed in the Enzyme, hence when titrated they dont meet the criteria to be classed as a even a "neat" so we report them as too weak to titre. 

Just wanted to check  if this is standard practice as in my previous experience all titres were sent to reference lab and I never saw any report that wasn't at least a "neat" 

 

Thanks

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  • Malcolm Needs
    Malcolm Needs

    We always used the last tube/column in a cassette that gave a macroscopic reaction. We certainly used "too weak to titre".

  • We don't ignore the "weaker" agglutinations -- we still record M reactions... however, the result of the titer is the last dilution that is graded >M (i.e. is graded 1+). We have a test result call

  • We use a titer value of 0 (zero) to indicate absence of agglutination or less than 1+ agglutination in any tube.

comment_78399

We always used the last tube/column in a cassette that gave a macroscopic reaction.

We certainly used "too weak to titre".

comment_78454

We don't ignore the "weaker" agglutinations -- we still record M reactions... however, the result of the titer is the last dilution that is graded >M (i.e. is graded 1+). We have a test result called "below titerable levels" for a titer that doesn't have reportable results similar to what you are describing.

comment_78455

We use a titer value of 0 (zero) to indicate absence of agglutination or less than 1+ agglutination in any tube.

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