Hi,

We have a Group A Pos patient antibody screen negative by capture. The 16 cell panel is reacting by IS (1-2) auto control negative. The patient was never transfused, not pregnant. How do you approach these cases other than running a SIAT panel at 37C? 

 

I must be missing something here.  Why did you do a 16 cell panel if the antibody screen was negative?  

21 minutes ago, John C. Staley said:

I must be missing something here.  Why did you do a 16 cell panel if the antibody screen was negative?  

I was wondering this too. 

Trouble with the reverse A cells?  We had a similar case recently.

Scott

It was part of the SOP to do IS panel if everything is reacting in reverse including screening cells and auto control negative.

18 minutes ago, Learning from the experts said:

It was part of the SOP to do IS panel if everything is reacting in reverse including screening cells and auto control negative.

We have had some colds, non-reactive in solid phase but reactive in tube. In above case, I would approach it by performing antibody identification by tube method. (since I am conservative) 

Edited April 6, 20196 yr by dothandar

I was thinking about a 5 screen panel by SIAT or GEL. If everything reactive I will set up some cord and group A and B cells .

 

1 hour ago, Learning from the experts said:

I was thinking about a 5 screen panel by SIAT or GEL. If everything reactive I will set up some cord and group A and B cells .

 

Sounds like a good plan, if you see "top line" in your gel. I would include an autocontrol when I set up A, B, Cord cells and extend incubation time till autocontrol comes up positive. (to really make sure that I am not looking at an allo-antibody) Hope you get your "rule-outs" in tube saline tube method! Good luck! 

Edited April 6, 20196 yr by dothandar

For the simular case we had, all we did extra was a 30 min, 37 C settle test (unspun) to resolve the positive reverse A cells (A patient).  We did not see a point in doing anything else--the gel screen was negative.

Scott