DCeDCe

3 homozygous cells, unless we are ruling out C or E in the presence of anti-D. In that case we use 3 heterozygous rule outs.

Sometimes the DAT can be negative due to destruction of the cells. Just because it is negative does not mean that there is not An underlying antibody present. Sooo, if your DAT is positive, regardless of the strength! you should perform the eluate.
Sometimes the DAT can be negative due to destruction of the cells. Just because it is negative does not mean that there is not An underlying antibody present. Sooo, if your DAT is positive, regardless of the strength! you should perform the eluate.

It doesn't mattrer if the patients antibodies are not showing. That just means that the titer has dropped off to undetectable levels. You should only allocate blood that is e, C, and K negative. DO NOT listen to Whitney poplin!!!! If you give units that are positivevfor those antigens, you can stimulate an immune response, Which will cause a transfusion reaction!!!

I'm sorry Whitney Poplin, but I disagree with your post.  Just because the antibody screen is currently negative does not automatically rule out anti-C and anti-K, for the very reason that it does not rule out the known anti-e; that is also not detectable at present.  From the logic of your post, you could, therefore, also rule out the known anti-e, and give e+ units.
 
No, the anti-C and anti-K should have been ruled out properly in the first place in my opinion.
 
Now, because this was not done, you would have to honour the potential anti-C and anti-K, in case either of these "phantom" antibodies cause a transfusion reaction, due to an anamnestic responce - and, of course, the same applies for the "real" anti-e.

In my lab, we are required to perform QC on the expired panels for the antigen that we are trying to rule out/in. When we do this, we are required to use a diluted antisera, as he manufacturer recommends his.

Flying squad?? Theater person?? I am lost by your lingo. Please explain.....

Why is your blood supplier even sending you those types of units? You cannot transfuse FFP from a donor who has an antibody, but aside from that, your blood d supplier should not be sending your facility those type of units. My old hospital had its own blood donation center, and we NEVER sent the other hospitals that purchased blood from us, units that came from a donor with an alloantibody.

In my lab, we are required to perform QC on the expired panels for the antigen that we are trying to rule out/in. When we do this, we are required to use a diluted antisera, as he manufacturer recommends his.