Hankct9

Thanks R1R2, It seems like the MTS diluent may have been bad. I repeated the gel XMs with freshly opened diluent and the XMs were negative with no hemolysis. I then repeated with the old MTS diluent and there was some hemolysis. The problem may just be with the diluent. Maybe I should have done complete testing before posting my problem..........oh well. Thanks again for your help.

The Immediate spin crossmactches were negative.

We have a patient who came in recently and required a unit of PRBCs. The patient is an A1 positive with a history of anti-K and LuA dating back to 1999. Today his/her AB screen was negative using gel(no hemolysis was seen). 2 units of A pos red cells were crossmatched using gel, per our protocol. To our surprise, both columns were completely hemolyzed. An AB panal was then done, which was negative, as was the poly and mono DAT. The patient was then gel crossmathed with O cells, which were negative with no hemolysis. Ultimatly the patient received one unit of o cells without incident. We can't figure out what's going on. Does anyone have an idea? Thanks in advance for any help. Hank