Hi , First of all I want to say that the members of this site are wonderful: I have learned so many things from yours posts. This is really the best forum of immunohematology on internet. I’d like to ask your help for a case. I have 2 patients, 2 brothers with the suspect of McLeod’s Syndrome on the basis of their neurologic symptoms . The neurologist ask to me the study of Kell antigens. They are both: K- k(cellano) + Kpa- Kpb + IAT: negative The serologic reaction of k( cellano) are 4+ with Immucor’s microplate technology and the serologic reaction of kpb are 3-4 + on Diamed’s column agglutination As I have read on this site, I perform a titration of kell antisera with patient’s cells and with blood donor’s cells. The patient’s titres are the same of the blood donor’s ones. On this basis, can I say that the probability of McLeod phenotype is not very high, also if I’m not able to demonstrate the absence of Kx? :confused: I’m sorry for my English and thanks in advance for any help
Effectively I am not able to demonstrate the absence of Kx because I haven’t Kx antibody and also in molecular testing in our BB we haven’t the specific primers for it (really I am not sure there are primers commercially available for Kx ). Thanks a lot for your help, Malcolm!!!!!!!!!! :):):):):):):):):):):)