Number 1 is WRONG as following this procedure will lead to possible anamnestic response*. Number 2 is the RIGHT PROCEDURE to follow to prevent possible anamnestic response*. Number 3: This is definitely NOT A TRICKY QUESTION. Anamnestic Response: An accentuated antibody response following a secondary exposure to an antigen. Antibody levels from the initial exposure may not be be detectable in the patient's plama until the secondary exposure, when a rapid rinse in antibody titre may occur. This may lead to haemolytic transfusion reaction.