K@The_Dresdeners

Thank you everybody. It is great to have someone to ask for help in such cases. :-)

Right,we just use the rbcs straight out of the segment. But in this case, the reactions with panel cells were 3+,so(even with a little less strong suspension) i would expect weak positive reactions at least. I did some tests with packed cells today-same results...negative in IAT crossmatch.

just open it with segment openers (sarstedt)

Panel cells are ok in quality control and with other patients. I tried to wash some BioRad cells 3x with NaCl and resuspended it in diluent 2.Same reactions. Segment preparation: 5µl of segment-rbcs, 500 µl of dil.2.

yes, same. column agglutination, BioRad gel cards 37°C for 15 min. incubated

my first thought was anti-H too, but in crossmatches with group 0 we had negative results.

I'm sorry. I can't speak (and write) english well. :-/ We had positive reactions with every cell in IAT, but none in IAT xmatches. Patients plasma was negative with 0, A1 and A2- cells,3+ with B-cells in NaCl.

Hello everybody, We can´t find out the antibody... Pts blood group is A1D CcD.ee K-, Anti-AB 4+, Anti-A 4+, Anti-B neg, Auto neg., Anti-A1(lectin) 4+ Anti-H neg. (backward: 3+ with B-Cells, negative with 0-, A1-, A2-cells) IAT, Papain and CaptureR screens are positive at 37°C/ 3+ with all cells, IAT screen at room temperature 4+ (gel column cards, BioRad and Grifols) Antibody identification was done in IAT 37°C (3+ with all cells), DAT weak+ IAT screen with washed cells 3+ But... in xmatch with RBCs from bloodbags (PAGGS-M): A1D CcD.ee K-, A2D CcD.ee K- and 0D CcD.ee K- all negative in IAT 37°C /gel column cards Can anybody help me out? Thanks, K.

Hello! Is anyone doing a platelet xmatch with Galileo(immucor)? How long can we use the platelets in a tube or bag for testing? As long as the "swirling" is still there an the QC is ok? Or do the platelets "expire" for testing after a couple of hours? We recently had a pt. with transfusion reaction, so we had to test/"xmatch" pt. an platelets in MAIPA- xmatch with "Leo" would be sooo much faster if we have some active/swirling platelets, I think. btw: Great site- thank you everyone out there! K.

When we have cases of AB0i renal transplantation we titre the patients plasma against known reagent red cells and against donor red cells by manual testing (gel-card,37°C IAT and 20°C NaCl) and Galileo (MTP for IgM and SC for IgG). Works fine. :-)

Hallo! Pt. with 0D CcD.ee K- DAT 3+( IgG3+,IgA-,IgM-,C3c-,C3d-,ctl-) plasma: 2+reactions with all e+ cells in panel eluate:Auto-Anti-e (acid-elution with BAG) needs RBCs, we should do an auto-absorption to get the auto-anti-e out of the plasma before we use it for xmatch to rule out an underlaying alloantibody, right? without absorption autocontrol is 2+, xmatched rbcs 2+ can anybody tell me a good (and simple,fast...) way to do an auto-absorption? seperate the plasma, wash the pt.cells with warm 0,9% NaCl- and .....?? we don´t have any other elution system than BAG, so we can´t use eluated cells to do the absorption. Thank you, K.

When the pt. will need more transfusions and we give rr red cells, could we try eluation 3 month after the last D pos.-transfusion, when DAT is still positive? I think she requires more RBCs in the next time, so we probably won´t have a complete 3 month transfusion-free interval while she is in our hospital. K.

Thank you:)! If there is an alloanti-D, would it look like an auto-antibody when the pt. was transfused with D pos RBCs in the past? K.

can a patient typed as weak D (type2) perform an Allo-Anti-D?