mpmiola

I want to start a discussion about the antigen and antibody of the ABO phenotypes. If subgroups are ABO that differ in the amount of antigen carried on red cells. By reducing the number of antibodies in the assay reaction will intensify?

Does anyone have any reference on the production of anti-A and anti-B, which cell or cell line is responsible?

Dear Malcolm, We did molecular analysis, which confirmed the presence of the O in the homozygous state. Interestingly, only one class of antibodies is not being produced. Thanks for contributing.

Diamond-Blackfan syndrome have depressions of production of anti-B antibodies and not anti-A? I have a case with direct typing "O" and reverse "B". Can anyone help with information and experiences?

The LW can also be confirmed after treatment with DTT. I agree with Malcolm Needs, I think this scenario an auto-anti-LW or auto-anti-D is more likely.

In our service we no longer use filter bedside, but we filter after storage, in bench for QA terms. I agree that pre-storage filter would be best, but still not able to implement it here.

I've used this technique successfully in detales see the article: "A quick and simple method for phenotyping IgG-sensitized red blood cellsT.S. SERERAT, D. VEIDT, AND A. DUTCHEDPositive (IgG) direct antiglobulin test (DAT) reactivity ranging from weakly positive to 2+ can be eliminated using a simple “blocking” technique with anti-IgG. This method can be used for antigen typing DAT-positive red blood cells that require the antiglobulin technique. Immunohematology 2000;16:154–156."

DogLover, I mean the leuko-redeuction filters, filters our service at the time of use. I need to know if you filter after 5 days of storage is effective?

Need to know how opinions on the use of filter RBCs stored for more than five days. Our stock phenotyped red cells is not filtered, so when necessary use phenotyped RBCs stored for more than five days for patients with sickle cell anemia, decided to wash red blood cells, performing the transfused with RBCs poor in leukocytes... How are you guys doing in these cases?

Thanks everyone ... I will continue studying this case. If anyone remember a new data, please email me.

According to the mother, not.

John, did not develop anti-D immediately after transfusion. The child was transfused with four units of plasma last year. We identified an anti-D two months later, that remains almost a year. Already excluded medications, Mabel ... I wonder publications about ... Thankful ...

Malcolm, we detected anti-D in a child who just received transfusions of plasma D +. I appreciate your attention and look forward to their research without worrying about the weather ...

Malcolm, we detected anti-D in a child who just received transfusions of plasma D +. I appreciate your attention and await their research without worrying about the weather ...

Antigenic particles dispersed in the PFC, can cause sensitization and development of anti Rh-D? Anyone heard anything or know of reports described. thank you!

Thank you Malcolm and Emily, sorry for the difficulty to understand the writing, but the important thing is that this forum has helped me in the completion of the case, especially the poster in PowerPoint.

"This might be done more easily by splitting her palsma sample into two, adorbing one with r'r red cells and the other with Ro red cells, and then by testing the aliquot adsorbed with r'r red cells against Ro red cells, and that adsorbed with Ro red cells, against r'r red cells.

This might be done more easily by splitting her palsma sample into two, adorbing one with r'r red cells and the other with Ro red cells, and then by testing the aliquot adsorbed with r'r red cells against Ro red cells, and that adsorbed with Ro red cells, against r'r red cells.

Dear Malcolm, I have the results are reported, the mother was discharged from the hospital and I have doubts on how to release the report, a likely anti-G is right, but how to demonstrate association with Anti-C and Anti-D? As for the baby, had progressive improvement after exchange transfusion with red cells rr, no doubt. Another question is how to release the report because depending on it may or may not be prophylaxis for future transfusions, likely think of releasing Anti-D, Anti-C and anti-G isolated after adsorption / elution.

HDN caused by anti-G+C or only anti-G? Results: Mother D-, sérum reacting whit D+ and C+ (Anti-DC), DAT negative. Following the technique of double elution with RBCs dCe/dce (D+G+C-) cells to isolate anti-C and -G, then this eluate, which contains the anti-D, is adsorbed onto and eluted from Dce/dce (D+G+C-) to isolate anti-G showed positive results only for D+ erythrocytes panel, not reacting with C+ the second eluate. Baby, D+, with Anti-DC serum (reaction 4+), eluate Anti-D (4+) but Anti-C (1+). Total bilirubin 7 hours after birth increased from 2 to 8. Hemoglobin dropped from 15 to 10. Questions? 1. What is causing HDN? 2. Why the Anti-G did not react with RBCs C+? 3. Why in the eluate drink no difference in intensity between reactions with erythrocytes D+ and C+? 4. Suspect Anti-G+C, but there opniões different in my service, with the exams presented it is possible to conclude?

Thank you so much.

Dear Malcolm, thanks for the help, you can tell me a reference for study?

Anti-LW can be exacerbated when tested with enzymatic panel?