mpmiola

Thank you Baby Banker. If cardiac surgery is for extracorporeal, should red cells be washed if they present more than 24 hours of irradiation time?

I also agree with the subgroup of A. However, I would do other tests before considering the case as resolved. 1. Are the reagents you used from different manufacturers from the same clone? Do they have the same title? Differences in clone or titer may lead to differences in reactivity. 2. Tn antigens may be cross-reactive with some anti-A antibodies. Treatment of RBCs with the enzyme may help to exclude this interferant. 3. I would or would refer you to the molecular investigation to identify the deletion mutation at position 261 of exon 6, which characterizes O alleles. 4. Flow cytometry can also assist in the evaluation of the mixed field. Is it happening because of poor reactivity or chimerism?

Hello, I need your help with transfusion of packed red blood cells in pediatric cardiac surgery. Our surgeon requests washed and irradiated red blood cells for all children up to three months old and for children over three months, only if it is the second procedure. Did we ask why? However, he could not answer. It is important to say that we provide red blood cells of 5 to 7 days of storage, only that is 3-5 days post-irradiation and identical ABO. I believe it has no significant benefits. Some studies say that washing minimizes the risk of inflammation, but it is not a consensus. Other risks seem to me to be more worrying than the benefits pointed out, such as: Risk of contamination - our service does not have automation for the washing of red blood cells; Loss of yield - in addition to the loss of cells that occurs during the procedure, mechanical trauma can accelerate the process of hemolysis of more fragile cells; Anyway, I would like to know your opinion on this subject and what is the practice adopted by your service? Thanks for any input,

We only issue units that are 3-5 days post irradiation. Thanks a lot

Thank you, I'll do it.

John C. Staley, he asks for all children up to three months old and for children over three, only if it is the second procedure. When we asked for a referral for this conduct, he did not provide it! I believe it has no significant benefits. Some studies say that washing minimizes the risk of inflammation, but it is not a consensus. Other risks seem to me to be more worrying than the benefits pointed out, such as: Risk of contamination - our service does not have automation for the washing of red blood cells; Loss of yield - in addition to the loss of cells that occurs during the procedure, mechanical trauma can accelerate the process of hemolysis of more fragile cells; Anyway, I would like to know your opinion on this subject and what is the practice adopted by your service?

Hello guys, I need your help with transfusion of packed red blood cells in pediatric cardiac surgery. Our surgeon requests washed and irradiated red blood cells. Did we ask why? However, he could not answer. Someone knows what's best, red blood cells washed or not washed? It is important to say that we provide red blood cells of 5 to 7 days of storage and identical ABO. Thanks for any input,

I agree with Ciff and the Auditor that the best way is to transport it with cold material. This is how we do with external institutions to our Hemotherapy service. However, our inpatients are prepared for transfusion prior to delivery of the bags. When the nurse arrives with the bag, it usually does not take more than 5 minutes to install. So I have doubt if it really is necessary. Thank you for your contribution. Despite the difficulties we have ... we want to provide the best to our patients. I apologize for my poor English.

Yes, expect room temperature! Not necessarily the 30 minutes. This is the point! If we follow the guidance of the auditor and carry out the transport Cold (1-10), we will have to wait a little. Why not consider the transport time in RT?

The same nurse who transports, performs the transfusion. We have records of exit and start of the transfusion.

Thanks for answering Cliff! Our hospital is also closed and temperature controlled (18-24). Our legislation allows for 30 minutes. However, I think if it is not transported on ice, the pouch can be transfused as soon as it is delivered. Otherwise, the nursing will wait 30 minutes. So it seems the same to me ... can you understand me? What do you think now?

Dear's, I work in a Transfusional Agency that serves several Hospitals. In the hospital we are installed, we carry the transfusion bags in boxes validated for transportation without refrigerant. We have established a time of up to 30 minutes for delivery (from the Agency to the patient). However, an auditor asked to transport in boxes with temperatures between 1 and 10ºC. I can not agree with this, although it is in a tropical region, with temperatures up to 35ºC, our hospital has refrigeration with temperatures between 18 and 24ºC. I believe we are correct, but I need to know how this transport happens in other services. Appreciate your help.

Malcolm I'm asking me. How can a human being be so wise? Thank you for sharing your wisdom. I learned a lot from this post. I hope to have the pleasure of meeting you in person one day!

Malcolm, thanks for participating. Yes, a case of SCT with ABO incompatibility. Do you know if the donor B cells can produce antibodies autonomous way and for an indefinite period in the receiver?

Anna Sorry, I have difficulty with English. There are mixed field reactions. I'm using gel-test. The DAT is negative. The eluate is also negative. How to explain?

Galvan. Thanks for contributing! The patient did not die. It is being watched since his transplant that took place 5 years ago. The anti-B antibody is that disappeared after two years of transplantation. What I think is expected and our doctor does not. You know for how log a donor lymphocyte can replicate the receiver, producing anti-B antibodies?

Dear friends, I ask for help in this case! Receiver: B + Donor: A + After 7 days of the transplantation we identify an anti-B in the receptor that remained detectable for 2 years. He disappeared three years. Today, five years after the doctor thinks the anti-B should be present. I think not. Who is right? I ask: 1 - How long does the B lymphocyte donor can replicate in the recipient and produce memory antibody? 2 - The syndrome passenger lymphocyte is for how long? 3 - No stimulus lymphocyte should not stop producing the antibody? Can someone explain this occurrence? Already, thank you.

You did absorption-elution using serum and red cell A, B and O? You can help confirm the antibody reverse ...

Our practice is 4 hours. Would like another option for not having to keep sending in syringe ...

I'm having doubts about the validity of the blood in the syringe for pediatric transfusions. We use a protocol, valid for 4 hours. What the best practicing...

I like this theme. I believe that the phenotypic expression may change for reasons unknown.

transfusion "O +", is not very risky when it becomes routine in several services in trauma care.We do this in the absence of "O-"

Without knowledge of the patient's ABO group certainly AB plasma. Medical protocol in the plasma be transfused after setting ABO patient while concentrates transfused erythrocytes.You have to reference the recent study, 1:1, quoted?

Yes, no blood products. But, as this product becomes dangerous?still ...The RBCs keep her waiting in the emergency room is an acceptable practice?Should not be in the transfusion service in the form of immediate dismissal?

I need your opinion on an internal protocol. The doctor wants liberemos 2 units of blood transfused O- to care for polytrauma, before the patient arrives at the hospital. Will install as soon as the patient arrives. He said he wants to reduce the infusion of plasma expanders because it interferes with the surgical procedures. Will certainly increase our consumption of O-. Plasma expander interferes with the surgery? What do you think?