mminhas44

We did it easily by putting the gel card centrifuge in a 37C dry air incubator along with all the consumables. We waited 30 minutes to get all up to temp then did the testing. Worked great.
 

I tried it several times when we were still gel users, but never successfully.

It can, but it is exceptionally difficult to get the air in the reaction well up to a strict 37oC, and "cold antibodies" sensitise red cells VERY quickly.

Sorry - I forgot the C.
As the frequency of C is about 68%, you can remove 2 of those three.  That leaves you with 1 unit
Anna

Very very roughly, and assuming your donors are mostly White European
If you start off with 100 units, approx. 50 will be O or B
Of those approx. 5 will be K+ - so you will have 45 units.
Of those, about 2/3  (so approx. 30) will be Fya+, so you will have 15 left
Of those about 80%  (about 12) will be Jka+
So, if you're VERY lucky, the remaining 3 will be compatible.
Good luck!!!!!

U need to exclude recent transfusion or carry over from previous sample ( although the levels mentioned is high for carry over)
wise if above mentioned are excluded to consider genetic testing and parental  screening 

Could be platelet clumps..

cells/microliter
 

Could you be more specific? Are you asking about units, or whether results need to be verified?
 

I saw a Blood Hound prototype 5-10 years ago.  I wish the FDA would work faster.  I think the technology looks very very hopeful. 

I just saw the Blood Hound at the AACC. It does digital images of all the WBC's, RBC's and Plts on a CBC. Per the manufacturer, it is capabel of detecting and counting RBC inclusions, including malaria, in RBC's. It looked impressive and is pending FDA approval. Check out their website.

Agree - also, what blood type is the mother?  From an operational stand-point you have a couple of options: Call the blood type "indeterminate" or "unable to determine" or call the baby AB.  Either way, we would suggest repeat testing at 4-6 months and include A1 lectin typing if discrepancy still exists.  In the meantime we would give group O red cells and AB plasma/platelets until resolved.

Agree - highly probably that the SD being used is not appropriate i.e. to large. 
Results should be distributed across the L-J chart, with approx. 1:20 results in the 2-3 SD space.
Suggest review local and published SD for analytes

It sounds like your SD is set too low.  Try recalculating it based on recent results.
Scott

I'm not an expert, but flow cytometry may be helpful to classify the lymphocytes (B-cell, T-cell, NK cells) and see if an abnormal immunophenotype exists.

I am not familiar with ISO 15189 standards--at a minimum you obviously have to follow them, along with manufacturer's requirements--but it seems to me...
...like you are being advised to validate a particular analyzer by comparing it to another exactly the same, which presumably was compared to another exactly the same, which was...
With any new method, you must show correlation with a previous method, in this case, your manual method.  Comparing it to an analyzer at another facility seems rather pointless.  (The exception being if the other facility is part of your system, and you use the same procedures and reference ranges).
You should also be doing a reference range study to validate your reference range, especially if there is significant variance in the correlation.  It has been our experience that automated methods correlate pretty well with the old manual ESRs.
Scott