mminhas44

Hi Can prewarm technique be done on gel cars for discrepant blood groups?

Could be platelet clumps..

I just answered this question. My Score FAIL  

Yes, I wish to know the units?

Hi, Hope you all are safe and healthy. How do we report automated WBC count run on sysmex XN-3000?

Hello every one, In hemoglobin chromatography (HPLC), we sometimes get small, insignificant peaks. Like Hb-S peak of 8.0, Hb-C peak of 7.5 (RT 180 secs). The only things which we do are to either retest with same sample or ask for fresh sample. Point is, the hemograms and histories of such cases have not been helpful up till now. Is there anything else which could be done?

Hi, Hope you are fine. My problem is: Which other hemoglobins elute in same window (same retention times) as Hemoglobin S in ARKRAY ADAMS A1c HA-8180T Analyzer? (P.S. it gives retention times in seconds.) Regards

They come wrapped in tissue paper, while the core biopsy comes in container with formalin.

For marrow smears and the peripheral smears accompanying them, we use manual Leishmann staining. The slides we receive from outside are plain glass slides, un- frosted, company not known.

Hi, We often receive unstained peripheral blood and marrow smears from other cities in the country. These smears do not stain optimally at our facility, even after manipulating stain timings. Therefore reporting these is problematic. We suspect that folks out there fix the unstained slides in alcohol before sending them to us. Is there any solution for improving the staining??

Hi, Is it OK if the LJ chart graph lies on mean consistently (ie without fluctuations) ? Regards

Hi, Posting after a long time. Hope all of you are fine. My problem is this: We have bought Sodium Metabisulphite form Merck company, previousl;y we were buying it from some other company (I am forgetting the name). With this new reagent, even patients with more than 50% hemoglobin - S on HPLC don't have a positive slide sickling test or the sickling disappears gradually over the day. Can anybody help me with this???

Hi Autoimmune disorders and chronic viral infections can also be the possibilities Saima

Hi, I work at the lab of a public sector teaching hospital. Recently we shifted from manual to automated ESR analyzer by Vesmatic, and discussed our case with our QA team as well as people from ISO 15189. We were advised to validate the analyzer from another vesmatic validated analyzer as we cannot compare apples with oranges (oranges in this case is the manual method). Regards

Hi, First, regarding weak positive DAT/IAT; we confirm them under microscope and use check cells with them. Our weak reactions are taken as less than 1+. And you are right about having 2+ reaction after adding check cells although i also dont remeber the source of the info. Anyways I am gonna check this out. About storage of coombs reagents and panel cells, we dont "warm" the reagents before use but just let them stand for couple of minutes to come to room temperature befroe testing. We use poly specific coombs reagent (IgG & anti C3d) so we only warm the coombs reagent if using pre warm technique. Hope this helps regards

what if a company does not provide a calibrator for their reagent/ instrument? are there any alternatives?

is there any thing like machine QC versus reagent QC?

can calibrators be made in-house?

currently we seperate one segment for each unit during processing and place it in glass tubes with donor ID #. these are retained for a variable period of time till after the unit is issued.

we do not use the unit for transfusion but perform elution studies and workout group

I agree with Bill.

smudge cells at our place is nearly synonymous with CLL cells

We have a repeated problem, I haven't seen it to occur so frequently at other places. Our slides, especially marrow and touch imprint slides, get understained. we have tried changing staining time, re-making stock solution but the problem is still there.Does anyone have any logarithm for it ?

what are treated slides and barrier slides used for ? can these be prepared in-house?

dear rravkin, it sure has made sense. Thanks a lot