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rscheuermann

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    rscheuermann reacted to galvania in Ortho C-D Gel card versus BioRad Gel Card   
    You make  it sound as though the bio-Rad system does not pick up Kidd antibodies.  I can assure you that just is not the case.  If it were, then there would be a huge number of transfusion reactions in hospitals using the bio-rad system - and that is not the case.
    There may be all sorts of reasons as to why these particular antibodies are not showing up.  First of all, can I suggest you try repeating manually.  As this is a trial, there may be a problem in the way the instrument has been set up - although that is a long shot.  Then, what I strongly suggest is that you contact your local bio-rad rep and let them send the samples to Switzerland.  Switzerland will need to know exactly which lot of cells and cards you were using, they will want print outs of the results from the IH1500 - and as much information about the age of the sample, whether it was frozen etc as you can give them.
  2. Sad
    rscheuermann got a reaction from jojo808 in Ortho C-D Gel card versus BioRad Gel Card   
    Thanks for your comments. I didn't want to bias any discussion. We are correlating the new BioRad IH500 using the new Diamed cards vs our current Ortho C-D Provue gel cards and there is poor concordance with Kidd antibodies, specifically anti-Jka. The Ortho gel are much more sensitive picking up weak to 2+ reactions while the IH500 gel is showing negative reactions. Anyone have any history or experience with Kidd antibodies on the BioRad Diamed cards specifically?
    We are not seeing an evening out of antibody strengths between the 2 cards. Do you think it is a huge concern then that the Jka specimens are non-reacting with the Diamed cards given the rare instances of the antibody in patients. Also, naturally-occurring Kidd antibodies are very rare. Further, there are multiple blood bank cards/methods in the region ie. Ortho gel, Grifols, Immucor, and we can view any of a patient's Kidd antibody history to provide antigen-negative red cells. 
    Thanks,
    Robert
  3. Like
    rscheuermann got a reaction from AuntiS in Ortho C-D Gel card versus BioRad Gel Card   
    Thanks for your comments. I didn't want to bias any discussion. We are correlating the new BioRad IH500 using the new Diamed cards vs our current Ortho C-D Provue gel cards and there is poor concordance with Kidd antibodies, specifically anti-Jka. The Ortho gel are much more sensitive picking up weak to 2+ reactions while the IH500 gel is showing negative reactions. Anyone have any history or experience with Kidd antibodies on the BioRad Diamed cards specifically?
    We are not seeing an evening out of antibody strengths between the 2 cards. Do you think it is a huge concern then that the Jka specimens are non-reacting with the Diamed cards given the rare instances of the antibody in patients. Also, naturally-occurring Kidd antibodies are very rare. Further, there are multiple blood bank cards/methods in the region ie. Ortho gel, Grifols, Immucor, and we can view any of a patient's Kidd antibody history to provide antigen-negative red cells. 
    Thanks,
    Robert
  4. Like
    rscheuermann reacted to galvania in Ortho C-D Gel card versus BioRad Gel Card   
    OK - to clear up some confusion  (apologies for it being so late in the day!).
    Ortho in Europe has glass beads.  Ortho in the States has gel which is similar to but not identical to the Bio-rad (ex-DiaMed) gel.  As the original question was about Kidd antibodies, I will stick to that.  You all know that Kidd antibodies love playing hide and seek.  BOTH techniques will miss some Kidd antibodies - but not necessarily the same ones.  So you might see some antibodies coming up in Ortho and not in Bio-Rad; and you will see others that do the opposite.  And by the way, Immucor will pick up Kidd antibodies that no one else does but it's not sure that these are real but might be artefacts caused by Paraben.  It is fair to say that some antibodies just 'prefer' one system over another.  But across the board, it evens out.

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