xyzcaa

GEL tech will not tell you anything about CAA and you cannot confirm this antibody is CAA without resorting to PEG/LISS. In case of PEG, for a typical CAA, your immediate spin will give you all positive reaction. At this stage, you may want to rule out roleaux just in case. Then you carry to AHG and you most likely get positive reactions. At this time, you switch gear to 1 hour prewarm or 2 hours strict warm (shaking tube, not centrifuging). You will get negative reaction at AHG. If still positive, you need cold adsorption. Also CAA will interfere your ABO typing and DAT. Prewarm red cells and reagents can fix. DAT IgG negative. use blood warmer if CAA showing.

1), one reference lab claims like this: because the new anti-E was identified this time which is not showing in 3 days ago workup, this is a delayed transfusion reaction. Note: pt transfused red cell 2 days ago, this time DAT negative. no elution done at reference lab due to neg DAT. 2) One prestigious author in AABB publication claims like this: delayed transfusion reaction occurs only if the new allo antibody shows in eluate. Which is right? I prefer to the 2nd.

If waa is not showing, there is no any benefit to give any special treatment. Instead, do electronic crossmatch. The reason is simple: No allo antibody seen because your gel or peg screen is negative. Give phenotype similar red cells only if you do not have time to do adsorptions to complete antibody workup and pt is in life threatening situation. This is a method should be reserved as the last resort if your reference lab cannot complete in time. Also, you most likely are not lucky enough to have these phenotype similar red cells even from a big blood center. Coordination with the nurse station is a key to prevent the last minute rush.

Dear Barb, Could you please send me a copy at xyzcaa@yahoo.com? Thanks!

As a med tech, I cannot afford to fly to and pay the fees to attend the SBB review session. I am not sure if any of you reqesting Barbara Pdf has got her response or not. If you got one copy, could you pls forward it to me at xyzcaa@yahoo.com? Thanks.

I will imagine these kits, if available, should be exclusive for WAA allogenic adsorptions. Also, does your facility work WAA in house? How you handle it? The more specific, the better. Thanks.

WAA problems accounted for most of cases we sent to IRL for workup. I am looking for following information and appreciate if you can help: 1). Any vendors can supply “kits” with three cells of R1R1 R2R2 rr? How much? Shelf time? FDA licensed? 2) Any vendor can apply ZZAP “kit”? Cost? Shelf time? 3) This is a wired question: Assuming RH antigen phenotypes of patients availably via monoclonal antisera, is it possible to get commercially prepared (two) reagents which served as allo-adsorption cells with Jka- and Jkb- , respectively? Thanks.

The link mentioned at AABB tech manual chap 4 (page 110, 2008) is not available. Anyway to retrieve it? http://www.cbbsweb.org/attachmnts/disaster_plan_0605.pdf Thanks.

100C or 100F? TYPO IN SOP?