Liz

This is interesting if you would like to read it: "ABO-mismatched transfusions are not over-represented in febrile non-haemolytic transfusion reactions to platelets" M. H. Yazer, J. S. Raval, D. J. Triulzi and N. Blumberg, online: 22 JUL 2011

Thank you Dave (the thanks button stopped working).

PS: I plan to write a book on my experiences and what I have seen, OMG , I dont really want to, but I have been asked to..... so I might when I find time.

I agree with you Brenda, BUT it depends on where you are geographically on planet Earth. There are places where people have been killed for less than that (This is the "Just for Fun " thread, hmmmm, but seriously people can get furious, lose it and maybe later regret their reaction to the truth). So, we are careful to NEVER say that there is the slightest doubt that the other lab was wrong. Yes, I will tell the doctor and the professionals, absolutely; but, not the family. I will live to regret it or just die of guilt!!!

Thank you Malcolm and Brenda for this great information!!

Oh, that is good to know, I shall check with Haemonetics. thank you!

ok thanks, I shall wait...

Dear Dave, To clarify, and please excuse my ignorance or if I sound thick: Running serum with known Abs: I would need 1:1 volume with the autos and allos, and then do the PeG adsorption, or just apply PeG to the Allos with ... with what RBCs? (remember this is stored serum for students). By "2 drops peg/2drops plasma" you mean 4 drops of the PeG adsorbed serum, right? My reagent cells are .8% so I have to get the 3%... just talking to myself here . You lost me (again) at "Wash one time and add 50uL cells to IgG card and spin". What test is this? "You will need to do elutions for IgG + DATs" why? what do I do then?? You said above that its useless to know the specificity of the RBC ags, so why use the Elu kit? Why do you do with your cells after you have destroyed the Kell ags with WARM ??? Its nice that you take my intelligence for granted its there but not yet for PeG. Thank you for accepting abuse Liz

Malcolm, do you have the reference please?

If you remember, can you send the reference please.

This does not fulfil the CAP requirement, it really does not confirm that it is from the correct patient.

Thank you Dave, you have really put me out of my suffering with this information. (...... at least for the moment until I face another wall; hopefully not).:flirty:

Important question to Peg adsorption users: how did you validate the PeG adsorption? pleeease. And do you still use, order, WARM (zzap) for anything? if yes what? Thank you.

What is the patient's age?

Hello Mabel, That sounds good, what is the brand name of your system? Thank you

I just saw the posts only today. Thanks for the input. The problem is that patients actually refuse a second draw (often an initial one!!!). I must assume that the Nurses and phlebotomists will be responsible.

Follow up: New patient: Ab ID and Panel and AC all 2+. Performed one adsorption with PeG and the AbSc is negative. I love it. BUT: I used pooled cells so I am repeating, also used 2:1 Serum/PeG to reagent cells. Question1: The reagent cells are diluted so is 2:1 ok? Question 2: how do I validate my work? (I was thinking of mixing in an anti-K, 1:1 from another patient, and testing for it after PeG adsorption, hmmm)

Hello KBB, Can you send me a copy of your printed crossmatch tag please: ek01@aub.edu.lb and what is the brand of your printer? Thank you, Liz

We give ABO group specific to children beacuse of their small blood volume: there is no dilutional effect on the antibodies. We give Rh (D) negative apheresis platelets to ladies who are Rh (D) negative and in the child-bearing age.

You should come and teach my students, you are very good and patient.

Nice! Malcolm.

I am sorry to hear that.

Ok here goes: patients are given (not in the country I am in!!) antibody cards to present at a future admission in case they need a transfusion. The card alerts the Blood Bank that this patient has these RBC antibodies so that they pay attention when preparing blood units. (these antibodies were formed due to previous transfusion or pregnancy, I dont know about naturally). These are a lot of antibodies and Belinda is Duffy negative (resistent to malaria), what is her ethnicity? Ok so how much did I get? and if its not an exam : speedy recovery to Belinda!

I don't, I meant to write "Oh my so, its an open book exam question??" I should have added the question marks. I did not want to wrongly accuse anyone so I was actually asking. sorry!! By the way, I caught a spammer and Cliff deleted his post!!! hehehehe !! I discretely pointed out to his signature. I am very proud of myself :juggle:

Yes, I guess I learn the hard way. I am waiting for a new sample as I understand that the Abs are weakened if stored with Peg. So, if I need to perform an Ab ID would that also be in tubes, and what would be the ratio of the volumes, 4 serum/PeG to 1 reagent cells? and at what dilution should the reagent cells be? Lots of question ....... sorry.....