Liz

I hope you enjoy it!!!!!!!

I absolutely agree with Donna and Elizabeth, if the unit is needed for another patient within the famous 3 days I use it; and then should there be a need for the first patient, I prepare once again but at no charge.

Let me answer my own question: genetically Oh individuals are homozygous for nonfunctional H (hh) and Secretor (sese ) genes, resulting in a complete absence of type 1 and type 2 chain H, A, B, and Leb antigens. Those individuals also type as either Le(a–b–) or Le(a+b–) caused by the absence of the Secretor (FUT2) gene. So Lea ican be formed, not Leb.

The H antigen is defined by the terminal disaccharide, fucose α1→2 galactose. Two different fucosyltransferase (FUT) enzymes are capable of synthesizing the H antigen, FUT1 (H gene), and FUT2 (Secretor gene). FUT2 or Secretor recognizes type 1 chain pre- cursors to form type 1 chain H and Leb antigens. So Malcolm regarding Leb where does the H (FUT1) gene come in?

Bombay, Oh phenotypes, can produce Lea and Leb, if they inherit Le and Se. If the H substance is present do the A, B (depending on the genes inhertied) and Le tranferases compete for the H antigen?

Lea needs the Le gene. Leb needs Le, Se gene. It doesn't need the H gene because Se froms an H antigen. Right? If the H and Se genes are inherited: do the A, B and Le transferases compete for it ?

The Components are as follows (unless something changed that I am not aware of) FFP (after thawing) Expiration, if issued as FFP:24 hours. Thawed Plasma Expiration: 5 days from date original product was thawed.

Very interested Malcolm, and Dr Coombs' picture is there!

Thank you for the explanation Malcolm and for the time you took writing it!!

We have been practicing this since ages and I never asked why :disbelief...I did look it up because I was ashamed to ask here such a basic question but didn't find anything...... Merci

Thank you Malcolm, that was fast and I needed the reply before noon !!! (Its 10:30am now). Am I lucky to have a friend like you, and this forum to turn to, or what?

Why is Anti-A,B reagent used to test O cells?

Nooooooooooooooo!! I can't believe it!!!! What!!??!!!!!

in vivo...

It is possible. I like the way you think!

Oh I am not ignoring you Malcolm, sorry about that. I just need to analyse what you said.

well, well well, lookie what I found: of the Dombrock blood group sysytem, Dob; and of the Rh blood group anti-e can cause delayed HDN. Why and how are to follow....

I agree that you can individualize, and decide as the need arises.

The fetus uses the mother's RES to clean up and once the baby is born, because of the immature immune system and RES that is when the bili etc shows. I am still wondering why the delay..

You do not have to run the Yta and Fya negative cell every time. You are keeping (archiving) the results and even if the antibodies are no longer detectable you still honor the history.

We follow OSHA reg, clogs are allowed as long as the they are completely closed in the front. The fear is from spills and other things that can fall. The Staff is asked not to run in case of emergencies but to walk calmly.

That is typical Blood Banking, and good practice and patient care. I am not sure I see where your concern is.

We ask them to administer synthetics and to send a blood sample until the AB FFP thaws. The double checked “red alert” O RBCs are immediately sent out, and that calms everyone.

I assume that the gel you are using does not have DVI+.

Certainly not, Mabel, we know that I even had to go back to your post to see what you were referring to.