mdcbk

I use the recipes below for students/new techs. We use mostly gel for testing, and these work pretty well most of the time. The Rh, K, and M are very consistent and we get 2+ reactions or better. I don't know what kind of reactions you would get with tube testing with the Rh and K. I've had problems with the Fy, so I only use it in combination with other antibodies. Sometimes I mix it up based on what our expired panels look like. If I'm feeling particularly evil (or want to stump a student who thinks they can solve anything), I will choose several antibodies that are very difficult to identify with a particular panel. We use mostly monoclonal antisera, and I try to use expired, but since I'm only using a drop or two, I don't feel bad about using in-date reagents. I either match the antibodies to an expired panel cell to use as "patient" cells, or use some segments from a phenotyped unit if one is available. I've tried making positive DATs with reagents other than D, but haven't had much success.


Elution: Anti- D, mixed field DAT
2 O Pos Segments and 2 O Neg segments
Put O pos segments in 12x 75 tube.
Add 1 drop of Anti-D, 2-3 drops of albumin and a little bit of saline
Incubate at 37 for 30 minutes
Add O neg segments

[*]Gel ab screen- Anti- C

Fill 12x75 tube 1/3 full of saline
Add 1 drop Anti-C, 2 drops albumin

[*]Gel ab screen- anti-Jka

Fill 12x75 tube 1/3 full of saline
Add 1 drop Anti-Jka, 2 drops albumin

[*]Gel ab screen- anti-K and Anti- c

Fill 12x75 tube 1/3 full of saline
Add 1 drop Anti-K, 1 drop Anti-c, 2 drops albumin

[*]Tube Ab screen – Anti-M

Fill 12x75 tube 2/3 full of saline
Add 2 drops Anti-M, 2 drops albumin

[*]Gel Ab screen - Anti-Fya, Anti-E, Anti-K

Fill 12x75 tube 1/3 full of saline
Add 2 drops Anti-Fya, 1 drop Anti-E, 1 drop Anti-K, 3 drops albumin

I use the recipes below for students/new techs. We use mostly gel for testing, and these work pretty well most of the time. The Rh, K, and M are very consistent and we get 2+ reactions or better. I don't know what kind of reactions you would get with tube testing with the Rh and K. I've had problems with the Fy, so I only use it in combination with other antibodies. Sometimes I mix it up based on what our expired panels look like. If I'm feeling particularly evil (or want to stump a student who thinks they can solve anything), I will choose several antibodies that are very difficult to identify with a particular panel. We use mostly monoclonal antisera, and I try to use expired, but since I'm only using a drop or two, I don't feel bad about using in-date reagents. I either match the antibodies to an expired panel cell to use as "patient" cells, or use some segments from a phenotyped unit if one is available. I've tried making positive DATs with reagents other than D, but haven't had much success.


Elution: Anti- D, mixed field DAT
2 O Pos Segments and 2 O Neg segments
Put O pos segments in 12x 75 tube.
Add 1 drop of Anti-D, 2-3 drops of albumin and a little bit of saline
Incubate at 37 for 30 minutes
Add O neg segments

[*]Gel ab screen- Anti- C

Fill 12x75 tube 1/3 full of saline
Add 1 drop Anti-C, 2 drops albumin

[*]Gel ab screen- anti-Jka

Fill 12x75 tube 1/3 full of saline
Add 1 drop Anti-Jka, 2 drops albumin

[*]Gel ab screen- anti-K and Anti- c

Fill 12x75 tube 1/3 full of saline
Add 1 drop Anti-K, 1 drop Anti-c, 2 drops albumin

[*]Tube Ab screen – Anti-M

Fill 12x75 tube 2/3 full of saline
Add 2 drops Anti-M, 2 drops albumin

[*]Gel Ab screen - Anti-Fya, Anti-E, Anti-K

Fill 12x75 tube 1/3 full of saline
Add 2 drops Anti-Fya, 1 drop Anti-E, 1 drop Anti-K, 3 drops albumin