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Quick search - ViaCord

We have Ortho Visions and do autoverify.

It is my understanding that the Ortho Vision is not 'licensed" for immediate-spin compatibility testing. (Licensed may not be the correct terminology) Please refer to Ortho published materials for immediate-spin eligibility.

We routinely reflexively test (and charge) for antithetical antigens for questions related to fetal risk for HDFN. We routinely perform an ABO/Rh type on specimens where only an antibody screen is ordered for a pre-natal patient (no charge if previous history). We routinely perform and charge for a DAT/ABO/Rh type/Antibody screen for antigen phenotyping for Daratumumab and hemoglobinopathy patients.

We are also experiencing this. My system isn't offering sign-on bonuses for technical staff but up to $30,000 for acute care nursing. Our lab has at least 12 vacancies on 2nd shift and similar for 3rd shift. 1st shift technical staff are working incentive contracts for bonuses - double shifts for some or weeks without days off - and are burning out so morale is at an all time low. Retention of staff is a big problem. My lab is staffed by long-term staff - all the recent hires (within last 4 years) have quit - gone to school, out of the profession completely, gone to other facilities that are closer to home and pay more, gone to other labs that pay more for less work stress. We just started hiring travelers for some of the technical positions and phlebotomy because there just aren't applicants for full-time, permanent positions and the pay just isn't enough and there are so many vacancies - definitely a buyer's market for lab staffing.

To wash less than 250ml RBC on the COBE/Terumo (I think at one point it was Gambro too), you will need to adjust the setting for Superout Volume and potentially increase volume of either saline or Plasmalyte A (either can be used) since the volume of the COBE bag is 650 ml. As the volume of RBC decreases, the volume of wash solution increases. Depending on the age of the COBE, it is either programmed electronically or with pegs.

I found this article today discussing the risks of transfusing blood with higher K+ levels in infants. MINI REVIEW article Front. Pediatr., 29 October 2021 | https://doi.org/10.3389/fped.2021.765306

Agreed - competency is very hard and we got through CAP Hybrid inspection this year and still got dinged because the review signatures on the summary page were not obtained within the competency testing period - competency due for 2020 signed in January 2021 even though the direct observations and testing were done within the 1 year period. Dismaying to say the least.

Our MTP protocol gives RBC and plasma through the Level 1 or Belmont and a second IV access is used for platelets

At my facility, the pharmacy and the Blood Bank collaborated in obtaining, storing, thawing, labeling and issuing the product. We did not have a standalone printer to create modification labels and our BB LIS considered it to be a pharmaceutical product. Pharmacy ordered the product after the patient's ABO type was available, shipped it to the BB for storage and thawing. Once thawed, the pharmacy picked up the product(s) and labeled the modified product and issued it to the patient infusion area. Fortunately, this has only come up twice in the last 8 years.

I can describe how it works for our healthcare system and PM a copy of our policy. The pre-hospital transfusions on the helicopter pre-dated me at this facility but I have expanded to ambulance pre-hospital transfusions on 3 ambulances with begging to increase to 4 ASAP. We maintain sets of 2 O NEG RBC and 2 low-titer liquid Group A plasmas for 1 helicopter and 2 ambulances that work out of the level 1 trauma center. We set up a dummy patient for each vehicle and units are pre-labeled for that dummy patient, segments are retained at the Blood Bank, each unit wears a Saf-T-Vue10 indicator and a triplicate Emergency Release form with unit numbers documented on the form with one copy of the form retained in the Blood Bank to keep track. Each vehicle uses the Pelican Biothermal Credo and changes out each blood product set once every 24 hours. The crew at the vehicle base is responsible for exchanging the cold panels for the credo. Each credo carries a datalogger and the datalogger report is downloaded and emailed to the Blood Bank at least weekly for review. Each credo is validated to maintain temperatures for up to 3 days prior to being released for active use. When a patient is transfused, the flight/medic crew is responsible for returning one of the copies of the emergency release form signed by the crew's MD to the originating Blood Bank and one copy goes on the patient's chart. Units are released from the dummy patient, crossmatched (if we get a sample), and transfused in the LIS to the actual patient. The crew returns to their base to replenish the units that were transfused. If the patient is transferred to a hospital that isn't part of our healthcare system, we still have a copy of the form returned to us and the crew will tell us where the patient was transported to and we "transfuse" the dummy patient with unit comments in the LIS with whatever information we received from the crew .

I suggest to my techs during training that it may be prudent to test the last wash before preparing the eluate - especially when additional red cells are not available. I'm sorry to report that I have seen several elutions where the last wash never became negative..... usually with cord cells where the mom had a VERY strong antibody - even changing tubes with every wash. I'm sure that if I had had the patience and persistence, it might have become negative but I gave up at 10 washes.

Our facility has built the attestation statement as part of the Emergency Released order set. We implemented this after our last FDA inspection in 2019 so don't have any feedback from them yet. We also use a triplicate form that documents the diagnosis, date and units that were issued during the process.

AABB Transfusion article from February 2019 discusses anti-CD47 interference and possible mitigation methods. It discusses how PEG adsorption may not be valid due to precipitation of the anti-CD47 which carries the risk of also precipitating out any alloantibodies rendering the tests negative (but invalid). It also discusses the blocking of antigen sites causing a negative or weakly positive DAT but a strong panagglutinin in the eluate. The conclusion was that multiple alloadsorptions using papain-treated red cells and use of Gammaclone anti-IgG may mitigate the reactivity. My question is whether anyone is using these methods for mitigation, were they successful, and how are you obtaining papain-treated RBC for the adsorptions? Can you use W.A.R.M (ZZAP) for the papain pre-treatment? I ask this because my facility is the 'reference' lab for a large hospital system but doing a 2-stage enzyme procedure is not feasible with my staffing and workload.

Change your setpoints to 2.5-5.5 - that way you are covered for both RhIG and blood products

Back in the 80's, had a cardiac patient undergoing CABG and received 8 units B NEG RBC during surgery. Three days later, a new specimen was received that typed mixed field AB POS with a reverse type group A. Supervisor retrieved the chemistry specimen from 3 days prior and it typed as A POS. Turns out the phlebotomist drew the patient in the other bed and this was long before second sample requirements .... Patient was a little "oozy" post-operatively but survived the experience. Same hospital, elderly female patient typed as O NEG. Blood was crossmatched and issued during the weekly computer downtime. As I went through the stack of units that had been issued updating the computer records, got a major flag as at computer issue - the unit was A NEG and that was just the first of 2 units that had been issued during this 2 hour downtime. Investigation revealed that 2 A NEG RBC were placed in the O NEG inventory, were crossmatched using immediate-spin and were compatible, and out the door they went. The patient was fine.

We use Pelican ProMed credos for carrying blood on ambulances and helicopters. To what extent to we need to go to re-certify these credos annually? Every payload includes a datalogger. Would it be acceptable to review temperature data during certain months, verify that the panels are within expiration dates and that the containers appear undamaged to consider them to be re-certified for another year?