jmm8427

Does anyone have a good SOP/training suggestions for use of 0.01M DTT? We don't use our SOP very often and when we do go to use it I found staff struggle a little bit with the whole thing. If you have any technique or procedure suggestions it would be greatly appreciated!

I love this! Thank you!

I would check your SOPs as to what to do next, but looks like you need to run a few more panel cells to rule in/out anti-C and anti-E. You can't just say the patient probably won't make the antibody, you have to prove that it is not there. Many places will allow rule outs with 3 D-C+c+ and 3 D-E+e+ cells.

We've started incorporating Quotient and BioRad products which are significantly cheaper and they work as well as Ortho and Immucor. Also monitor your reagent supply (like ABO/Rh) and make sure you are not expiring a lot and you are ordering what you can use. Making your own controls can help a lot, too!

Is it at 50, 000 yet?

Rita, We use the Ortho MTS monoclonal gel cards (C, c, E, e) and take the controls right from the 4% panel cell vials. We only use the Diluent 2 Plus to dilute if we are using patient or Donor cells to test. Hope this helps!

For the Bombay-Long day and I can't remember, is Lewis not expressed too? Cromer was the system on DAF

wow had to go to the books for this, Kpc was reported in 1945. very devious Malcom! I hope that's right! What system is located on the complement regluatory glycoprotein DAF (decay accelerating factor ) ?

I'm a newbie with a question- One place that I used to work would perform a 1 hour saline-IAT incubation after Gel/PeG technique screens were all positive if a warm autoantibody was suspect. If they could get that screen negative they would just call it the warm auto, if any screen cells were positive they would look for underlying allos. If it was all still positive they would send the sample to their reference lab. They did this to get around adsorptions, since they did not do them. There is a procedure in the AABB tech manual for 2 drops of plasma, but they would use 4 drops. Sorry so long, but my question is if anyone else uses this technique, and it's different than what the AABB tech manual has- is there any data to back this method up? Thank you!