marquij@caribe.net

What would be considered acceptable criteria???

Mycoplasma pneumonia in adults (anti-I) and Infectious Mononucleosis in children (anti-i) are the most common conditions?

Agree with Malcom: Crossmatch compatible if anti-M not reacting at 37C. Confirmed antigen negative if anti-M reactive at 37C.

Got a question to post: Which antigen is associated with the development of peptic ulcers and stomach cancer?

I think this is it!! Novotna M, Havlicek J, Smith AP, Kolbekova P, Skallova A, Klose A, Gasova Z, Pisacka M, Sechovska M, Flegr J (2008). "Toxoplasma and reaction time: Role of toxoplasmosis in the origin, preservation and geographical distribution of Rh blood group polymorphism" Still trying to figure out a question to post.

At our facility an autocontrol is tested with all antibody ID's if the antibody screen is positive.

We only check microscopically the cord cell DAT's.

Forgot my question: What is used to neutralize Sda antibodies???

McLeod syndrome patient rbc's lack KX and have depressed expression of all Kell blood group system antigens.

At our facility we do the 24 hr outdate for thawed FFP. If TFFP is not used after 24 hrs; we modify it in our computer system to the 5 day expiration product, print new labels with the new product information and label the product accordingly .

We perform PeG autologous adsorptions at 37C for 15 mins adding equal amounts of plasma/PeG/adsorbing cells. For testing 4 drops of adsorbed plasma. Works well and if suspicious of underlying alloantibodies, may test with 6 drops to avoid missing any weak reactivity. The problem with PeG according to the literature is that it precipitates proteins and since antibodies are globulins thay may precipitate and be missed.

No ficin treatment of the adsorbing cells prior to adsorption at 4C? This will enhance the adsorption process and less adsorptions will be needed.

We store our specimens for 14 days in individual racks labeled with the date. At midnight, the night shift tech disposes of the samples that are 14 days old and labels that rack for today.

We don't routinely wash red cells, unless requested by the physician. This would most likely be a preventive measure or recommended for patients that have experienced allergic or febrile non-hemolytic reactions in the past.

Best of luck! I am getting ready to challenge the exam hopefully by the end of this year.

We also do cell separation by microhematocrit centrifugation, but we use plastic tubes for safety which are easy to cut using regular scissors.

We honor the previous identified antibodies and only do a selected panel every 72 hrs to detect any new antibodies.

Two homozygous whenever possible to rule in and out..

Whatever specificities present in plasma are detroyed by ficin treatment of the rbcs. This may be the reason for unsuccesful stromal adsorptions. Maybe adsorptions with untreated red cells will help isolate/elute the specifity, identify underlying aby unless you have an low avidity aby in such case you will not be able to adsorb it out. Crossreactivity with bacterial antigens may be the reason of these hit and miss rxns. More info: diagnosis, tx history, etc might be helpful.