trisram

can you send me too? rsnowayinhell1@gmail.com

You're correct, IAT means indirect coombs test, not indirect antiglobulin test ... Sorry, I forgot what IAT meant. I see what you mean. Sorry, I usually work in microbiology. I am no blood bank expert.

No. Direct coombs, DAT, in vivo. I don't know anything about IAT .

I apologize. We don't use IAT here where I work, just the direct coombs. But anyways, I was out of line. I apologize

I apologize, I was out of line.

nevermind. thanks. what kind of panels you use? 11 cell panel, all O Positive cells? or do you use select cells too? any other reagents? thanks, but this doesn't answer my question one bit

your answer doesn't make any sense. where are you getting all these cell samples? so I get both positive and negative cells? what does that prove? can i just expired panel cells as select cells? I just want to rule out everthing but my two suspected antibodies. that's it. I don't need all these other rubbish

Thank you, but what do you mean, 2 from cells samples? where can I get these samples? from the cell sample store? or can I use prior expired panel cells that are positive/negative for the corresponding antigens?

3 is not a question

1) You get a postive antibody screen. 2) You then do an antibody ID panel. 3) The results of the panel makes you suspect 2 or more antibodies in the patient's plasma. What do you do next and what reagents/methodology do you use? Thank you in advance for your time and help!

I wasn't trying to make decisions on you. I apologize if it seemed that way. I am just tired of incompetent people working in the medical industry. Enough patients have died already. Nothing against you personally because I don't know anything about you. But, anyways, I apologize, so sorry.

error

I don't care about diluents. This still doesn't answer my initial question. Dude, you're a SBB, do you know the answer?

Thanks but you misunderstood what I said. The washing with warm saline procedure was from the people who makes the gel cards. It wasn't for replacement, because there was no plasma to replace and no antibody to wash away. We add the plasma afterward. Well, this is what happened: 1) We did the gel card Ab screen and it showed rouleaux in the gel. 2) we read manufacturer's procedure of ridding rouleaux by washing screening cells 6 times 3) after washing screening cells, we pipet them into the gel cards and finally add the patients plasma 4) incubate for 15 mins 5) centrifuge gel card 6) Rouleaux all gone My question is , why wash the screen cells? what are we washing off? forget the plasma. this is not replacement. we add the plasma after we wash our screen cells with warm saline? why warm saline? why not cold? like I said, if Malcolm doesn't know the answer, chances are, nobody does

Wow, if Malcolm doesn't know the answer, it probably doesn't exist