Lindz82

We order from Sigma Aldrich.

Thanks!

Okay I am wondering what types of ernzymes everyone uses... we currently use Ficin/ Papain, and are looking at some others... What are your thoughts?

Hi ALL! Does anyone have a good resource for alpha- chymotrypsin. I need a recipe and treatment procedure. I appreciate any help you can give! Thanks!

Thank YOU!

Hello All! I currently work at an IRL to be in the midwest. We have a current ABO discrepancy SOP, but to be quite honest it needs some work. I was hoping I could locate a couple of GREAT SOPs, and I know you guys have them, so I can start reworking our unorganized, confusing mess.... Thanks!

In addition to determining reactivity at 37, you can also treat the serum/plasma with 0.01 M DTT to destroy IgM reactivity and determine the presence of an IgG componet... I know many hospitals don't do this but reference labs usually can. (Note this procedure is mostly used for determining the class of Mama's Aby, at least here it is)

Would bacterial "Junk" also cause to eluate to turn up positive?

She is caucasian. Has recieved multiple transfusions. The stroma we use for adsorption is not enzyme treated. I am unsure about other diagnostic information except that sepsis is probable. Could sepsis cause this kind of extra reactivity?

I have a case study i would like some input in .... be forwarned I have no definitive answers and that is why I am here... Patient is a 95 year old female. Hospitalized for amputations. Original antibody ID (10-22-09) Found an Anti-K with PeG in tubes.Extra reactions at AHG in gel. Auto was + at AHG in gel. Eluate was -. Thought to be a drug interaction of some sort.... Last week we worked up patient again and found Plasma had reactions varying from w+ to 3+ in gel, only Anti-K in PeG. Ficin diminished extra reactions. Extra reaction were DTT resisitant. Reactivity was not inhibited using normal pooled plasma. Eluate is now + w+to 2+ some negative cells in both plasma and eluate. Performed a stromal alloadsorption using R1, R2, and r stroma and extra reactivity was not adsorbed out of plasma but was adsorbed out of eluate. I will try to provide more info if it is asked for. Thanks for any input.

Thanks for the input Malcolm. I just have a hard time telling a client hospital it is probably RhIg when it is reacting 3+... I worry it is allo-anti-D and I may lead tham astray...

In maternal specimens...Out of curiosity... How strong can RhIg be (in gel column)? How long after dose is given will it still show up? Is there any way to ensure that it is actually RhIg and not Anti-D? Thanks for the Info! Lindsay

We currently do molecular typing... on all donors and some patients. Its saves time, but must be confirmed in order to be considered Antigen negative! (at least until it is licensed by the good ole FDA)

I am finding this site addicting... I am kind of a blood bank nerd and love reading about all of the many many things i did not know. I am not calling you all old.... Just some of you:-P

In my Ref lab we offer the clients choice... Antigen negative units (tested and tagged) and Hist Neg units. If the client hosp chooses Hist neg units... to save money... it is their responsibility to confirm hist neg typings. We do not confirm ag types on hist neg ... that would just be ag neg blood which the client must request.

That is just policy for us... I am still a newbie so I am quickly learning no two places are the same. (It also means I don't heve much say in policy... yet:tongue:)

Yes Malcom... I agree. The specific point I was getting at was the cold auto. If we see the reactions we HAVE to at least attempt to ID it.

Hi! I am from springfield, IL. I am currently working at the Central Illinois Community Blood Center in the Refernce Lab. I graduated in 2008 and look forward to learning so much from blood bankers that have seen more than i have..... (and there are a lot of you out there)!

Thank you so much! I will definately shoot him an email.

I work at a reference lab becoming an IRL..... We are currently diving into doing drug induced hemolysis work-ups! HELP! Does anyone have any advice or experience about creating drug treated cells or any other procedure related....

i work in a reference lab ( though not yet an IRL). Two things... 1. As a refernce lab it is our job and duty to identify and specify ALL antibodies in a specimen. That is the reason it is sent to us. So we use both gel and tubes. Test at 37 degrees and RT and cold if needed. We are providing the most complete picture we can for the client hospital. 2. Why in the world would you wnat to send out a specimen to a reference lab that could not even repeat the reactivity you see!! That souds nutty to me. ... I guess I have three things...3. i have personally seen up to a 2+ differennce in sensitivity between gel and PEG or LISS. That means a 2+ SIGNIFICANT antibody could be missed if only using tubes! I know that there is some extra pain in the rear reactions in gel... but isn't it worth it for the sensitivity that could help the patient!

Bear with me as I am new not only to bloodbanktalk but Blood bank in general. I am trying to decide if it is worth the trouble to make our own complement check cells. Currently we use Gel for Anti-C3b,-d testing and the reactivity of check cells is much improved from tubes. My main curiosity is are homemade check cells worth the trouble? The money it will save. That's what it is all about... right? Please share your adventures in cooking up reagents!