:wink: I asking for the best protocol/technique for acquiring a platelet count for platelet rich plasma samples. I was recently left without a reason why four samples sent for analysis were returned with four extremely different platelet counts. The four samples were all from the same 5cc sample. Two analysis' used a 4:1 dilution technique and the other two were direct aspiration. All four samples should have yielded at least a 3X baseline but did not. The fifth sample sent from the same batch, analyzed without dilution yielded a 8X baseline which was very close to our calculated goal. I don't usually question the results but I would like to know if there is a best way to do this, as I am not a lab guru and I don't want to go back to counting them by hand. Thanks! -Rick