khalidm3
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Saudi Arabia
Everything posted by khalidm3
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B Sub Group
A blood donor revealed no reaction in forward: Anti A= 0 Anti B=0, Anti AB=0 Anti D= 0 Control= 0 Reverse A cells= 4+ B cells= 0, O cells=0 (Room temperature and 4 C) We performed adsorption elution by using plasma from Blood Group A donor. The elute produced 3+ reaction with B Cells and Control elute negative. What to write donors blood group?
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Positive DAT on neonatal sample.
Thanks-D,
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Positive DAT on neonatal sample.
Thanks Auntie and Malcolm Some times we do not have mother's sample so we just use neonate's sample. Units compatible-with baby sample by AHG. Yesterday I had a similar case, so posted it here.
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Positive DAT on neonatal sample.
For pre transfusion compatibility testing , What is the importance of positive DAT on a neonatal sample whose ABS(3 cell panel) is negative whereas all neonatal transfusion are O red cells.
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Releasing RBC units post surgery
We return the units to inventory next day to operation day unless treating physician or nurse inform blood bank to hold the units due to low Hb or some other reasons. It is mentioned in our return blood policy.
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IS Crossmatch with Gel IgG Crossmatch
A few of our staff practice the same though it is not according to our SOP. Long ago I was on the bench and was practicing the same; My supervisor objected that I was wasting the reagents and I replied to him that if he not allow, I may buy the reagents from pocket but will not changethe practice. He kept silent and never objected again.
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Use of Coulter Lyse S III diff lytic reagent to lyse reds in urine
Did u validate? U counted cells pre and post lyse?
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Look Back for HBV
Thanks Profbuad, I have set Syphilis=One year, HIV, HTLV= Indefinitely, HCV 10 years, HBsAg and HBc for 5 years. I will discuss with my medical supervisor and may change HCV indefinitely also. Thanks for pointing to 15th edition.
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problem with leishman staining
25 years back, I was in a general lab and faced this problem. I remember if the stain was prepared according to recommended concentration 150gram powder in 1000 Methanol; we we facing similar problem. I remember we we preparing a saturated solution of Leishman stain and were getting ideal results for smears.
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Look Back for HBV
Thanks David, It will be in both cases, if a donor is reactive and we have to look back his previous donations or the a patient get TTD/TTI due to transfusion. The policy is required by CAP TRM.47320 Donation Tracking
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Look Back for HBV
What is the period for Look Back for HBV (HBsAg) and for NAT: HBV, HCV and HIV
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Centralized temp monitoring
Oceasoft data loggers has capacity for storing data for 3 months, each instrument has a data logger with unique serial number, if serve or wireless, net connection is down, the data from loggers is transferred as soon as the system is available. The thing I donot like till now are probes that connected to data loggers by wires, not wireless transmitters. On start of complete system I may know other things.
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Centralized temp monitoring
We are installing Oceasoft (Cobalt), They have probed sensor, can be dipped in glycerol-water but wired to data loggers, recording temp every 15 minutes. The date transfer from data loggers is wireless to a server computer, may send email and SMS and has a wireless hooter. We included Refs, Freezer, platelet incubators and room temp/humidity. New experience, not yet completely installed. We will change four hourly readings to daily one reading. Is it required daily one reading? We will set a 1 C acceptable variation between two systems and have set alarms/hooter at 1.5, 5.5 (Refs), -25 (freezers) and 20,24 for incubators. Please Comment I shall share my experience after a couple of months.
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high hemoglobin as a cause of deferral
At our center, upper Hg level is 18, to avoid risk of a Polycythemia rubra ver transfusion and dehydration risk for donor. It is the decision of our medical director.
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Disaster experiences shared?
Excellent listThanks
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Go though radiation room
Thanks
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ASCP certification
More than USA, for USA citizens having USA qualifications, Tax free
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ASCP certification
Here in Riyadh KSA, some hospital pay extra for US certifications but mine none- I did not get any benefit since 1998, but I am maintaining it (ASCP)CM) which benefits me to keep my knowledge up to date
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Go though radiation room
I am not in USA, but here our hospital is getting USA accreditations, Like Moc serve for JCI in June and final in Nov 2011, and soon there is CAP and mostly AABB, also. So we are changing our facility according to AABB/CAP/CLIA requirements.
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Radiation Detractor
Thanks, We detects radiation leakage by batches, but I want to install an instrument which may hoot as soon as there is a leakage.
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Go though radiation room
Yes, it is cesium
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Go though radiation room
Sorry Clif, My question is not about security, Is it? I shall be careful in future.
- Radiation Detractor
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Can patients with Weak D antigen develop anti-D?
Term Weak D is applied to AHG test (Du), not to direct Rh test. If the reaction in direct test is less than +2 by manual or less than +3 by DiaMed Cards, we should further evaluate/trace that it is not from FMH or Rh in compatible transfusion. Week D test is not required to perform for patients other than new born. So if a pregnant female is direct Rh negative, and her husband/baby is Rh positive or week positive, she is a candidate for RhoGm. We perform week D (AHG) for our all our patients and donors and inform them their actual Rh status, Rh Negative, Rh Week Positive or Rh Positive accordingly.
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Go though radiation room
There is no leakage of radiation from the unit. It is closed, during radiation process also and we do not walk though during the process.