skyanto

I was preparing a pooled Platelets from Buffy coat. I noticed these yellow things in a buffy coat. Any suggestions? Thank you.

just for curiosity... in my lab when we have to release blood components technicians -receive blood's request -choose blood components -perform compatibility tests -confirm results in the computer system -issue units for transfusion Physicians -sign blood's requests (but only during the day and sometimes after the issuing of the blood products... ) -sign group reports This only if all compatibyty tests are negative. If there is a positive test we have to call physicians. How is in your labs? :osorry for mistakes

Hello everybody, thanks for waiting us but it is very difficult to speak at work... We've thought this explanations: 1- patient is A and she has little title of anti-B ( we had a similar case a few weeks ago) *diamed NaCl card at 4°C *50microL plasma +50 microL A1,A2,B,O red cells *30' incubation 2-patient is AB with a weak B antigen (something like ABx or ABm ) *adsorption and elution method *adsorption: plasma patient (with anti-B )+ donor red cells B and then elution. *adsorption: donor plasma A (with anti- + patient red cells and then elution. 3-bone marrow transplant -patient is AB and she has been a transplant with bone marrow or cordon cells type A (find patient history) 4- we don't know...maybe severe immunodeficiency ; so patient is A but she hasn't antibodies... Sorry for our english..Buen trabajo a todos

Hello Malcom I've read your "nice little problem" yesterday. Por favor could you wait until next week to give us the four explanations? I would like to talk about it in my lab and then try to answer ... Gracias

From mistakes we have to learn.. and in this case I've learned that my procedure isn't correct and I don't understand why. I've checked the neutralization after 1 hour at 4°C and the plasma was neutralized so in my plasma there were only IgG...

Finally today I've done the anti-A (IgG) titer of the ABO incompatible platelet. We use five buffy-coat donors to do a platelet unit so I've done the titer on the five donors plasma in this way: 100 microL NEUTRAB+100 microL plasma donor 1 hour at 4°C I've checked the neutralization with 1 drop A1 cells+1 drop neutralized plasma if no agglutination I've diluited the netralized plasma 1/2, 1/4, 1/8 until 1/512 in diamed cards liss/coombs 50microL A1 cells+ 25 microL diluited plasma 1/2, 1/4... 15 minutes at 37°C centrifugation Results: 4 donors were negative,titer < 1/2 1 donor was positive,titer >1/512 BUT I've done a mistake...I put the cards in the centrifuge without incubation at 37°C ...I've repeted the test in the correct way and I've obtained the same results. The positive result is not correct because what I saw are IgM and not IgG... This method is not right ..Next week I'll ask the correct procedure. Anyway from the "day two" of this case the patient didn't riceive any transfusion. The last time he came for a visit ha had Hb 8.2, PLT 25000, LDH 583 and BILI TOT 1.52.

When we have a positive DAT we test the patient red cells with a Diamed card called DC SCREENING I.In this card there are this monospecific AHG reagents: anti-IgG, -IgA, -IgM, -C3c and -C3d.We had a positive reaction (2+) only with IgG so the only antibodies on the red cells of this patient are IgG.At the moment I'm not at work and I don't know nothing about the patient...I was curios about the procedure to do the IgG titre but in my lab there isn't the DTT reagent we only have a reagent called NEUTR-AB that we use to neutralize IgM and then titre the IgG in mom 0 with A or B child...Maybe I can use this reagent to do a test but only for curiosity... Buen fin de semana a todos.

We've tested the eluate with A1 and B cells. The results are: positive 4+ with A1 and negative with B. So the DAT is positive because in the group 0 PLATELET there were IgG anti-A in high titer.Do you have a procedure to do this titer? Muchas muchas gracias a todos.

History: Mr. C.O. is a 63 years-old male with lymphoma.He is a politransfused patient. Day one: Hb 7.8, PLT 3000, Bili.tot 2.13, Bili.Ind. 1.82. Type and screen: DAT negative, IAT negative, Control group A+. Transfused with 2 units of RBC (A+) and 1 unit of PLATELET (0+). All units were irradiated and filtered.Chills at the end of the transfusion. Day two: Hb 8.2, PLT 10000 ,Bili.tot 2.31, Bili Ind. 1.98, LDH normal. Type and screen: DAT positive 2+(diamed card), IAT negative, Control group A+,Monospecific : only IgG positive 2+ (diamed card), ELUITION negative( gamma elu-kit immucor and diamed panel 11 cells). We have repeted the Day-one DAT and IAT and it was all negative. We have cross-matched the two RBC units and they were compatible. We have done a DAT of the two units and it was all negative. We repeat on a new sample DAT and IAT : DAT positve 1+ IAT negative. Transfused with 1 unit of RBC and 1 unit of Platelet. Why the DAT is positive after 24 hours? Gracias and sorry for my english...

I remember only one case.

In my lab we do the subgroups A1 and A2 to all new donors and we report them A1 or A2.If we have a donor or a patient A2 with anti-A1 we report the group A2,the alloAb anti-A1 and we put in a special field a warning message.

Thank you very much Galvania :) I've read the "decreto" and I've found a lot of interesting informations.. I printed it this morning at work and all my friends were interested on it.. Hello ckcheng im my laboratory we haven't written guidelines about T&S..we started wiyh T&S at the end of 2008.For us T&S is perform a DAT,IAT and a control group;it's valid for three days and if a patient needs more transfusions during the three days we do only a control group. We have a lot of patients with DAT positive.. ckcheng what are SOP? BUEN FIN DE SEMANA A TODOS

Muchas muchas gracias for all yours replays:) This morning I put this result in my lab donors program: A2B. It's not a Aint because in Aint both reactions with anti-H and anti-A1 are positives, if I remember correct anti-H 3+ and anti-A1 2+ ( I found this in technical manual). I don't know nothing about AbantuB group.. I agree with Galvania : it's a boring old A2B..

I don't know...I don't know who decide in Italy.. I have to ask to my head physician.... tomorrow I'll ask to him.. muchas gracias Rasmi.

In my lab this morning we have a new donor from Kenya.To a new donor we determine the subgroup if the group is A.I have done the group and the subgroup with Galileo and the results are: -group AB -subgroup NTD (not determinated) negative reaction with anti-A1 and ANTI-H. I have done the subgroup with DIAMED cards: 50 microl. of red cells and 25 microl. of anti-A1 and anti-H,10 minutes at room temperature and 15 minutes centrifugation. The same results..negative reactions.. I have done it in tube: 2 drops of anti-A1 and ANTI-H and 1 drop of red cells, 10 minutes at room temperature and 1 minute centrifugation. The same results ...I have left the tubes at room temperature for abut 1 hour and then I have looked at the reactions ..under a microscope the reaction with the anti-H was positive.I left the tubes at 4°C for about 15 minutes and under a microscope the reaction wiyh anti-H was a little bit much positive... Is it correct to say that the new donor is a A2B subgroup?Muchas gracias