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DebBB

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    DebBB got a reaction from SMILLER in What are your rules for ruling out?   
    In my lab we follow the ANZSBT guidelines as shown below
    http://www.anzsbt.org.au/publications/documents/PLP_Guidelines_Mar07.pdf
    2.1.3.5 The specificity of an antibody [including each individual antibody in multiple antibody mixtures]
    can normally be assigned when it is reactive with at least two reagent red cells carrying the
    corresponding antigen and non-reactive with two reagent red cells lacking the antigen.
    2.1.3.6 The presence of anti-Jka, anti-Jkb, anti-S, anti-s, anti-Fya and anti-Fyb should be excluded using
    red cells with double-dose expression of the corresponding antigens.

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