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You must follow the package instructions for the anti-sera and it clearly states storage at 1-10C.  I don't care if you are doing QC on previously frozen antisera and if I was your CAP inspector you would get cited for improper handling of reagents because your procedure must state how to handle reagents and you would have to document that the storage is in the freezer.
I recommend you stop this bad practice.

I have used both the 2 and 3 cell Ortho screen and I personally love the 3 cell screen for several reasons. the Main reason is if you have an Anti-D you will have 1 cell negative to rule out some antigens. The same reasoning goes for multiple antibodies. If you have 1 negative cell to begin ruling out, then you can select cells that are homozygous for the remaining cells on your panel and decrease the time spent doing a complete panel. Any money save using a 2 cell panel will be spent in Tech time.

The Illinois chapter of ASCLS will be hosting a 2 day review seminar in April 2014.  I facilitate in an SBB program and there is more to the Exam than the Technical manual.  You need to have some Coagulation, Lab Management and Lab math books too or you will fail.

Anti-M does have both IgM and IgG components. It primarily reacts as IgM but can carry over to IgG. We had one last week. The IgM component caused an ABO discrepancy by reacting with with M+ A1 reverse cells in an A Pos patient. The IgG component showed up at coombs phase after the addition of Anti-IgG. We had to transfuse M neg units to have compatible cross matches.

you could use a specimen from Hematology that is drawn at a different time for your retype.  The policy should be for all patients and not a certain age.  Most preemies and babies get O Neg so then your policy could address that they only need 1 blood type if they are receiving O neg or a quick heelstick if you are doing type compatible.

You must follow the package instructions for the anti-sera and it clearly states storage at 1-10C.  I don't care if you are doing QC on previously frozen antisera and if I was your CAP inspector you would get cited for improper handling of reagents because your procedure must state how to handle reagents and you would have to document that the storage is in the freezer.
I recommend you stop this bad practice.

The "30 minute" rule used to be included in the Technical manual years ago but then was taken out.  If you have CAP accreditation, there is a standard that asks about your return policy.  it must be validated.  So we took several units of red cells over the course of a week and left them on the counter at RT taking the inital temperature and temp every 5 minutes with a calibrated thermometer and discovered the units warm up to over 6 C after 15 min so we updated our policy and sent memos to Nursing and OR about the new rule change.  10 C is too high, that is transport temperature and taking a unit to the floor does not fall under that category.  The unit should not go above your storage temp of 6 C.

Great case scenerio.  Thanks for sharing this with us.  I have had a weak Anti-Jka before and since it appears that you had a newly forming antibody, I might have repeated it with 4 drops of plasma and extended the incubation period for 30-45 min.  We have a policy that if the C3 becomes positive that we do the panel manually with polyspecific AHG and that is how we found our weak Anti-Jka.  I plan to share this with my senior CLS students.

Great case scenerio.  Thanks for sharing this with us.  I have had a weak Anti-Jka before and since it appears that you had a newly forming antibody, I might have repeated it with 4 drops of plasma and extended the incubation period for 30-45 min.  We have a policy that if the C3 becomes positive that we do the panel manually with polyspecific AHG and that is how we found our weak Anti-Jka.  I plan to share this with my senior CLS students.

Did you look at "agglutination" under the microscope?  Since its weak, it sounds like possible Rouleaux to me

The Illinois chapter of ASCLS will be hosting a 2 day review seminar in April 2014.  I facilitate in an SBB program and there is more to the Exam than the Technical manual.  You need to have some Coagulation, Lab Management and Lab math books too or you will fail.